MAB2150
Anti-E-Selectin Antibody, clone P2H3
clone P2H3, Chemicon®, from mouse
Synonyme(s) :
ELAM-1, CD62E
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Source biologique
mouse
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
P2H3, monoclonal
Espèces réactives
human
Fabricant/nom de marque
Chemicon®
Technique(s)
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
Isotype
IgG1
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... SELE(6401)
Description générale
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface, members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNF alpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NK kappa B dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
Spécificité
Human E-Selectin
Immunogène
IL-1(1 activated HUVEC (human)
Application
Anti-E-Selectin Antibody, clone P2H3 detects level of E-Selectin & has been published & validated for use in ELISA, FC, IP, IC, IH.
Immunohistochemistry: 1:50 – 1:500; acetone or paraformaldehyde-fixed frozen and formalin-fixed paraffin-embedded tissues; recommend heat-induced epitope retrieval in EDTA, pH 8.0 for paraffin-embedded tissues
Immunofluorescence
Flow Cytometry
Immunoprecipitation
ELISA
Blocking: 10-15µg/mL; inhibits carcinoma and monocyte adhesion to IL-1β activated HUVEC
Optimal working dilutions must be determined by end user.
Immunofluorescence
Flow Cytometry
Immunoprecipitation
ELISA
Blocking: 10-15µg/mL; inhibits carcinoma and monocyte adhesion to IL-1β activated HUVEC
Optimal working dilutions must be determined by end user.
Forme physique
Format: Purified
Purified by protein G and stored in 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl containing 0.05% NaN3.
Stockage et stabilité
Maintain at 2-8°C for up to 12 months from date of receipt..
Remarque sur l'analyse
Control
Ductal breast cancer or mormal colon
Ductal breast cancer or mormal colon
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
H E Young et al.
The Anatomical record, 264(1), 51-62 (2001-08-16)
This study details the profile of 13 cell surface cluster differentiation markers on human reserve stem cells derived from connective tissues. Stem cells were isolated from the connective tissues of dermis and skeletal muscle derived from fetal, mature, and geriatric
Preparation of chitosan films using different neutralizing solutions to improve endothelial cell compatibility.
Qing He,Qiang Ao,Yandao Gong,Xiufang Zhang
Journal of Materials Science. Materials in Medicine null
Natalya V Narizhneva et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 19(9), 1158-1160 (2005-04-19)
Expression of cell adhesion molecules (CAM) responsible for leukocyte-endothelium interactions plays a crucial role in inflammation and atherogenesis. Up-regulation of vascular CAM-1 (VCAM-1), intracellular CAM-1 (ICAM-1), and E-selectin expression promotes monocyte recruitment to sites of injury and is considered to
Enhanced expression of alpha(1,3)-fucosyltransferase genes correlates with E-selectin-mediated adhesion and metastatic potential of human lung adenocarcinoma cells.
Martin-Satue, M, et al.
Cancer Research, 58, 1544-1550 (1998)
Preparation and characterization of chitosan-heparin composite matrices for blood contacting tissue engineering.
He Q, Ao Q, Gong K, Zhang L, Hu M, Gong Y, Zhang X
Biomedical Materials (Bristol, England) null
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