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Key Documents

MAB201B

Sigma-Aldrich

Mouse Anti-Rabbit light chain Antibody, biotin conjugate

Chemicon®, from mouse

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.46

Source biologique

mouse

Niveau de qualité

Conjugué

biotin conjugate

Type de produit anticorps

secondary antibodies

Clone

monoclonal

Espèces réactives

rabbit

Fabricant/nom de marque

Chemicon®

Technique(s)

ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable

Isotype

IgG1κ

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Description générale

Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and gamma light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.

Spécificité

Minimal cross-reaction with bovine, goat, Armenian hamster, horse, human, mouse, rat, and sheep.

Application

Mouse anti-Rabbit light chain Antibody, biotin conjugate is an antibody against Rabbit light chain for use in ELISA, FC, WB, IH.
Western Blotting: 1:20,000 - 1:400,000
Immunohistochemistry: 1:500 - 1:5,000 on tissue sections
Flow Cytometry: 1:200 - 1:1,000

Optimal working dilutions must be determined by the end user.

Description de la cible

25 kDa

Forme physique

Lyophilized from 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 0.05% Sodium Azide. 15 mg/mL BSA as stabilizer (IgG-Free, Protease-Free)

Stockage et stabilité

Maintain lyophilized product at 2°-8°C for up to 12 months. After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. Reconstitute vial with of distilled water (addition of 0.5 ml water will yield a final concentration of 1.1 mg/mL). For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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N Rampersaud et al.
British journal of pharmacology, 167(7), 1467-1479 (2012-07-11)
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