MAB1254
Anti-Chymase Antibody, clone B7
clone B7, 2.4 mg/mL, Chemicon®
Synonyme(s) :
Mast Cell Protease I
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
B7, monoclonal
Espèces réactives
human
Fabricant/nom de marque
Chemicon®
Concentration
2.4 mg/mL
Technique(s)
dot blot: suitable
immunohistochemistry: suitable
Isotype
IgG1κ
Adéquation
not suitable for Western blot
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... CMA1(1215)
Description générale
Mast cell chymase (EC 3.4.21.39), a member of the granzyme subfamily of the S1 family of peptidases, cleaves C-terminal to Phenylalanine, Tyrosine, Tryptophan, and Leucine residues (in order of preference). Chymase is stored in Mast cell granules within the cell and is the major secreated Mast cell protease. Secreated Mast cell chymase is believed to be involved in extracellular matrix degradation, vasoactive peptide generation, and regulation of gland secreation.
Spécificité
Reacts with human mast cell chymase.
Immunogène
Chymase purified from human skin.
Application
Anti-Chymase Antibody, clone B7 is an antibody against Chymase for use in DB, IH.
Dot blotting: 0.5-1.0 μg/mL.
Immunohistochemistry: 0.5-1.0 μg/mL. For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.
Not recommended for use on Western blots.
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 0.5-1.0 μg/mL. For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.
Not recommended for use on Western blots.
Optimal working dilutions must be determined by end user.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms
Inflammation & Autoimmune Mechanisms
Forme physique
Format: Purified
Purified by protein G affinity chromatography. Liquid in PBS with 20% glycerol, pH 7.4.
Stockage et stabilité
Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt. Antibody may also be stored at -20ºC in undiluted aliquots.
Remarque sur l'analyse
Control
POSITIVE CONTROL: lung, heart, skin, placenta.
POSITIVE CONTROL: lung, heart, skin, placenta.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Co-cultivation of mast cells and Fc epsilon RI alpha+ dendritic-like cells from human hip bone marrow.
D Kaur, P Berger, S M Duffy, C E Brightling, P Bradding
Clinical and Experimental Allergy null
M Yamada et al.
Kidney international, 59(4), 1374-1381 (2001-03-22)
Mast cells (MCs) are known to participate in various types of chronic disease, but their role in chronic renal rejection is poorly understood. Recently, distinct phenotypes of MCs have been described in humans by the demonstration of one protease, chymase.
Buffy coat preparation is a convenient source of progenitors for culturing mature human mast cells.
X S Wang, K H Yip, S W Sam, H Y A Lau
Journal of Immunological Methods null
Mast cell regulation of human endometrial matrix metalloproteinases: A mechanism underlying menstruation.
J Zhang, G Nie, W Jian, D E Woolley, L A Salamonsen
Biology of Reproduction null
Fixation with Carnoy's fluid reduces the number of chymase-positive mast cells: not all chymase-positive mast cells are also positive for tryptase
KleinJan, A. et al.
Allergy, 51, 614-620 (1996)
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