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LIF2010

Sigma-Aldrich

Leukemia Inhibitory Factor from mouse

10 µg, mouse recombinant LIF protein, expressed in E. coli, suitable for stem cell culture

Synonyme(s) :

LIF, Differentiation-stimulating Factor, D Factor, Mouse LIF, Mouse Leukemia Inhibitory Factor, Murine LIF, mLIF

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About This Item

Code UNSPSC :
12352202
eCl@ss :
32160405
Nomenclature NACRES :
NA.75

Source biologique

mouse

Niveau de qualité

300

Pureté

>95% (HPLC and SDS-PAGE)

Forme

liquid

Activité spécifique

≥1 x 10(E8) U/mg

Fabricant/nom de marque

Chemicon®

Concentration

10 μg/mL

Technique(s)

cell culture | stem cell: suitable

Impuretés

<0.1 ng/mg Endotoxin (of LIF)

Entrée

sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)

Numéro d'accès NCBI

NM_008501

Numéro d'accès UniProt

P09056

Conditions d'expédition

wet ice

Application

RECOMMENDED QC PROTOCOL

M1 Bioassay

1. The M1 bioassay is performed using in vitro semi-solid agar cultures, which contain approximately 100 cells in 1 mL volumes of DME containing 20 % FCS in 0.3% agar.

2. Add 100 μL of sample or mLIF (10(E4) units/mL in 5% FCS in isotonic saline) in two-fold serial dilutions in duplicate to 35 mm petri dishes.

3. Add 100 μL of 5% FCS in isotonic saline to two control slides.

4. Incubate at 37°C in fully humidified atmosphere of 10% CO2 in air for 7 days.

5. Score the number of colonies that show differentiation (note: 50 units is defined as the amount of activity which results in 50% of the colonies being differentiated).

Visit www.esgro-lif.com for additional information

Manufactured by CHEMICON International, Inc. LIF is protected under US Patent nos. 5,187,077, 5,427,925, 5,443,825, 5,750,654 and 6,261,548, European Patent no. 0285 448 and related foreign patents and is not available for resale.

Définition de l'unité

Specific Activity: where 50 units is defined as the amount of mouse LIF required to induce differentiation in 50% of the M1 colonies in 1 mL agar cultures.

Remarque sur l'analyse

Specific Activity: The activity of mouse LIF is determined by the ability to induce differentiation of murine M1 myeloid leukemic cells.The minimum detectable concentration of mouse LIF in this assay is 0.5 ng/mL
Tested negative in both aseptic and microplasmic tests.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Yuanji Lin et al.
Molecular cell, 48(4), 627-640 (2012-10-09)
Signaling via the Akt serine/threonine protein kinase plays critical roles in the self-renewal of embryonic stem cells and their malignant counterpart, embryonal carcinoma cells (ECCs). Here we show that in ECCs, Akt phosphorylated the master pluripotency factor Oct4 at threonine
Kay Jüngling et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 17(14), 2100-2102 (2003-09-23)
The pluripotency and high proliferative capacity of embryonic stem (ES) cells (1-3) makes them an attractive source of different cell types for biomedical research and cell replacement therapies. A major prerequisite for these applications is the availability of a homogeneous
Tomoko Ishibashi et al.
Neuron, 49(6), 823-832 (2006-03-18)
Myelin, the insulating layers of membrane wrapped around axons by oligodendrocytes, is essential for normal impulse conduction. It forms during late stages of fetal development but continues into early adult life. Myelination correlates with cognitive development and can be regulated
K Kami et al.
Muscle & nerve, 22(11), 1576-1586 (1999-10-08)
Using in situ hybridization histochemistry, we characterized the spatiotemporal gene expression patterns of leukemia inhibitory factor (LIF) and glial cell line-derived neurotrophic factor (GDNF), and their receptor components (LIFR, GFR-alpha1, RET) induced in muscle cells, intramuscular nerves, and motoneurons in
M K Carpenter et al.
Experimental neurology, 158(2), 265-278 (1999-07-23)
The isolation and expansion of human neural progenitor cells have important potential clinical applications, because these cells may be used as graft material in cell therapies to regenerate tissue and/or function in patients with central nervous system (CNS) disorders. This
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Protocoles

Mouse Embryonic Stem Cell Culture Procedures & Protocols

Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.

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