CBL1337-I

Anti-PECAM-1 Antibody, clone 390

clone 390, from rat

• Synonyme(s) : Platelet endothelial cell adhesion molecule, CD31, PECAM-1
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Source biologique: rat
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 390, monoclonal
• Espèces réactives: mouse
• Technique(s): flow cytometry: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable; neutralization: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_032842
• Numéro d'accès UniProt: Q08481
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Pecam1(18613)

Description

Description générale

Platelet endothelial cell adhesion molecule (UniProt Q08481; also known as CD31, PECAM-1) is encoded by the Pecam1 (also known as Pecam, Pecam-1) gene (Gene ID 18613) in murine species. PECAM-1 (CD31) is a 130-kD cell adhesion molecule that is expressed at the junctions of all continuous endothelia, as well as most bone marrow-derived hematopoietic cells, including platelets, monocytes, granulocytes and some lymphocytes. The human PECAM1 and murine Pecam1 genes both produce multiple spliced isoforms with varying lengths in cytoplasmic domain, with the isoform Long being the major form expressed in human tissues and endothelial cells (ECs), and isoform delta 14-15 being the predominantly expressed murine form. PECAM-1 plays an import role in maintaining vascular integrity and function by mediating ECs interactions with pericytes and smooth muscle cells (SMCs). PECAM-1 is initially produced with a signal peptide (human a.a. 1-27, murine a.a. 1-17) sequence, the removal of which yields the mature protein with a large extracellular region (human a.a. 28-601, mouse a.a. 18-590) composed of six C2-type Ig-like domains, followed by a transmembrane seqment (human a.a. 602-620, mouse a.a. 591-609) and a cytoplasmic tail (human a.a. 621-738, mouse a.a. 610-727) that plays an essential role in PECAM-1-mediated cellular signaling.

Spécificité

Clone 390 immunoprecipitated the characteristic 120-130 kDa protein from L-cells transfected with muPECAM-1∆12,15 and selectively stained murine vessels in acetone-fixed tissue sections (Baldwin, H.S., et al. (1994). Development. 120(9):2539-2553).

Immunogène

Epitope: extracellular domain

Mouse leukocyte cell line 32D.

Application

Flow Cytometry Analysis: A representative lot detected the expression of wild-type, ∆12,15 and ∆14,15 murine PECAM-1 constructs on the surface of transfected mouse L-cells (Baldwin, H.S., et al. (1994). Development. 120(9):2539-2553).

Immunofluorescence Analysis: A representative lot localized PECAM-1 immunoreactivity by fluorescent immunohistochemistry staining of murine embryo cryosections (Baldwin, H.S., et al. (1994). Development. 120(9):2539-2553).

Immunoprecipitation Analysis: A representative lot immunoprecipitated the 120-130 kDa exogenously expressed PECAM-1 ∆12,15 construct from transfected murine L-cells (Baldwin, H.S., et al. (1994). Development. 120(9):2539-2553).

Neutralizing Analysis: A representative lot inhibited the aggregation of murine L-cell transfectants expressing wild-type and ∆12,15, but not ∆14,15, murine PECAM-1 constructs (Baldwin, H.S., et al. (1994). Development. 120(9):2539-2553).

Clone 390 is also available as FITC conjugate (Cat. No. CBL1337F) for direct immunofluorescent staining in flow cytometry, immunocytochemistry and immunohistochemistry applications.

Research Category
Cell Structure

This rat monoclonal Anti-PECAM-1 Antibody, clone 390, Cat. No. CBL1337-I is validated for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, and Neutralization applications.

Qualité

Evaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis: 2 µL of this antibody detected PECAM-1-positive monocytes among one million mouse splenocytes.

Description de la cible

79.49/76.14/78.23/68.05 kDa (mature isoform 1/2/3/4), 81.26/77.92/80.00/69.82 kDa (isoform 1/2/3/4 pro-form) calculated.

Liaison

Replaces: CBL1337

Forme physique

Format: Purified

Protein G purified.

Purified rat IgG2a in PBS without azide.

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable