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Sigma-Aldrich

Goat Anti-Mouse IgG Antibody, (H+L) Alkaline Phosphatase conjugate

1 mg/mL, Chemicon®

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.46

Source biologique

goat

Niveau de qualité

Conjugué

alkaline phosphatase conjugate

Forme d'anticorps

affinity purified immunoglobulin

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Espèces réactives

mouse

Fabricant/nom de marque

Chemicon®

Concentration

1 mg/mL

Technique(s)

ELISA: suitable
western blot: suitable

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Description générale

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
The reagent is an affinity purified antibody from goat. The purified antibody is conjugated to alkaline phosphatase and stabilized in buffer.

Spécificité

Specific for mouse IgG, heavy and light chain. The cross-reactivities of anti-mouse IgG antibody are tested in an ELISA. Minimum cross-reactivity to human IgG.

Application

ELISA: 1:3,000 (1,2)

Immunohistochemistry/immunocytochemistry: 1:200-1:500 (3,4)

Western Blot, chromogenic: 1:200- 1:500 (1,2)

Western Blot, chemiluminescent: 1:2,000-1:5,000 (1)

Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
This Goat anti-Mouse IgG Antibody, (H+L) Alkaline Phosphatase conjugate is validated for use in ELISA, WB for the detection of Mouse IgG.

Forme physique

ImmunoAffinity Purified

Stockage et stabilité

The undiluted antibody is stable at 2-80C for 12 months. Do not store in a diluted format. DO NOT FREEZE.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Transcriptional regulation of livin by beta-catenin/TCF signaling in human lung cancer cell lines.
Dong Yuan,Liqun Liu,Dayong Gu
Molecular and Cellular Biochemistry null
Amber Haynes Fradkin et al.
Journal of pharmaceutical sciences, 98(9), 3247-3264 (2009-07-02)
Aggregation of recombinant therapeutic protein products is a concern due to their potential to induce immune responses. We examined the immunogenicity of protein aggregates in commercial formulations of recombinant human growth hormone produced by freeze-thawing or agitation, two stresses commonly
Ayse Inhan Garip et al.
Acta biologica Hungarica, 61(2), 158-167 (2010-06-04)
It is by now accepted that extremely low frequency electromagnetic fields ELF-EMF (0-300 Hz) affect biological systems although the mechanism has not been elucidated yet. In this study the effect of ELFEMF on the number of apoptotic cells of K562

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