ABT242

Anti-Galectin-4 Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Galectin-4, Gal-4, Antigen NY-CO-27, L-36 lactose-binding protein, L36LBP, Lactose-binding lectin 4
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): primate (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), feline (based on 100% sequence homology), monkey (based on 100% sequence homology)
• Technique(s): immunohistochemistry: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_006140
• Numéro d'accès UniProt: P56470
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... LGALS4(3960)

Description

Description générale

Galectin 4, or GAL-4, is another member of the Galectin family. Galectins are carbohydrate binding proteins that appear important in cell-cell contact, cell migration, and cell to cell signaling in both normal and disease (cancerous) tissues. Galectin 4 differs from its sister family members in the range of carbohydrate substrates that it binds. Galentin-4 is synthesized as a cytosolic protein, but can be externalized. During development and in adult normal tissues, galectin-4 is expressed only in the alimentary tract, from the tongue to the large intestine. It is often found in relatively insoluble complexes as a component of either adherent junctions or lipid rafts in the microvillus membrane, and it has been proposed to stabilize these structures. Strong expression of galectin-4 can be induced, however, in cancers from other tissues including breast and liver. Also, Galectin-4 appears to specifically bind CD3 on activated T-cells in the gut and regulate their activity via calpain mediated apoptosis.

Immunogène

Epitope: near N-terminal

KLH-conjugated linear peptide corresponding to human Galectin-4 near the N-terminal.

Application

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

This Anti-Galectin-4 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of Galectin-4.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Galectin-4 in 10 µg of Colo320 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Galectin-4 in 10 µg of human small intestine tissue lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Galectin-4 in human stomach tissue.

Qualité

Evaluated by Western Blotting in HT-29 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Galectin-4 in 10 µg of HT-29 cell lysate.

Description de la cible

~36 kDa observed. Uncharacterized band(s) may appear in some lysates.

Forme physique

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable