ABT170
Anti-alpha Tubulin Antibody, nontyrosinated
serum, from rabbit
Synonyme(s) :
Tubulin alpha-1A chain, Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-3 chain
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
serum
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
mouse, porcine
Réactivité de l'espèce (prédite par homologie)
rat (based on 100% sequence homology), human (based on 100% sequence homology)
Technique(s)
ELISA: suitable
immunocytochemistry: suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... TUBA1A(7846)
Description générale
Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
Spécificité
This antibody detects the nontyrosinated form of alpha Tubulin.
Immunogène
KLH-conjugated linear peptide corresponding to human nontyrosinated alpha Tubulin.
Application
Detect Tubulin using this rabbit polyclonal antibody, Anti-alpha Tubulin Antibody, nontyrosinated validated for use in western blotting, ELISA & ICC.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Western Blotting Analysis: A representative lot from an independent laboratory specifically detected nontyrosinated alpha Tubulin and not tyrosinated alpha Tubulin from purified porcine alpha Tubulin (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).
ELISA Analysis: A representative lot from an independent laboratory specifically detected nontyrosinated alpha Tubulin, and not nontyrosinated alpha Tubulin in an indirect ELISA and in a competitive ELISA (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory specifically detected nontyrosinated alpha Tubulin, and not nontyrosinated alpha Tubulin in TC-7 cells in interphase (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).
ELISA Analysis: A representative lot from an independent laboratory specifically detected nontyrosinated alpha Tubulin, and not nontyrosinated alpha Tubulin in an indirect ELISA and in a competitive ELISA (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).
Immunocytochemistry Analysis: A representative lot from an independent laboratory specifically detected nontyrosinated alpha Tubulin, and not nontyrosinated alpha Tubulin in TC-7 cells in interphase (Gundersen, G. G., et al. (1984). Cell. 38(3):779-89.).
Qualité
Evaluated by Western Blotting in NIH/3T3 cell lysate, which were untreated or treated with Pancreatic Carboxypeptidase (PCA).
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected alpha Tubulin, nontyrosinated in 10 µg of PCA treated NIH/3T3 cell lysate and demonstrated a loss of signal in untreated NIH/3T3 cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected alpha Tubulin, nontyrosinated in 10 µg of PCA treated NIH/3T3 cell lysate and demonstrated a loss of signal in untreated NIH/3T3 cell lysate.
Description de la cible
~51 kDa observed
Liaison
Replaces: 04-1117
Forme physique
Rabbit polyclonal serum in buffer containing serum with 0.05% sodium azide.
Unpurified
Stockage et stabilité
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Distinct populations of microtubules: tyrosinated and nontyrosinated alpha tubulin are distributed differently in vivo.
Gundersen, G G, et al.
Cell, 38, 779-789 (1984)
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