ABS530

Anti-CDC7 Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Cell division cycle 7-related protein kinase, CDC7-related kinase, HsCdc7, huCdc7
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): rhesus macaque (based on 100% sequence homology)
• Technique(s): western blot: suitable
• Numéro d'accès NCBI: NP_003494
• Numéro d'accès UniProt: O00311
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CDC7(8317)

Description

Description générale

CDC7, also known as CDC7-related kinase or HsCdc7/HuCdc7, and encoded by the gene CDC7/CDC7L1, is a critical kinase needed to phosphorylate critical substrates that regulate the G1/S phase transition and DNA replication. CDC7 is a serine-threonine kinase that forms a complex with either DBF4 or DBF4B proteins leading to the activation of the kinase activity necessary for its function. Additionally, CDC7 phosphorylates and forms a complex with MCM2 and MCM3 proteins which are highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. CDC7 is localized to the localized in the nucleus, and CDC7 is over expressed in many tumors and transformed cell lines.

Immunogène

KLH-conjugated linear peptide corresponding to the protein kinase domain of human CDC7.

Application

Anti-CDC7 Antibody detects level of CDC7 & has been published & validated for use in CDC7.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Qualité

Evaluated by Western Blotting in human spleen tissue lysate.

Western Blotting Analysis: 1 μg/mL of this antibody detected CDC7 in 10μg of human spleen tissue lysate.

Description de la cible

~63 kDa observed

Forme physique

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable