ABS2230

Anti-USP46

• Synonyme(s) : Deubiquitinating enzyme 46, EC:3.4.19.12, Ubiquitin carboxyl-terminal hydrolase 46, Ubiquitin thioesterase 46, Ubiquitin-specific-processing protease 46
• Code UNSPSC : 12352203

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Poids mol.: calculated mol wt 42.44 kDa; observed mol wt ~42 kDa
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): mouse
• Conditionnement: antibody small pack of 100
• Technique(s): ChIP: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgGκ
• Séquence de l'épitope: Unknown
• Numéro d'accès Protein ID: NP_001127695
• Numéro d'accès UniProt: P62068
• Température de stockage: -10 to -25°C
• Informations sur le gène: human ... USP46(64854)

Description

Spécificité

This rabbit polyclonal antibody specifically detects USP46.

Immunogène

FLAG-tagged, full length recombinant protein of USP46.

Application

Quality Control Testing

Evaluated by Western Blotting in lysates from USP46 (KO) 293T cells and wild type 293T cells.

Western Blotting Analysis (WB): A 1:1000 dilution of this antibody detected USP46 in lysate from wild type 293T cells, but not in USP46 (KO) 293T cell lysate.

Tested Applications

Western Blotting Analysis: A representative lot detected USP46 in Western Blotting applications (Joo, et al. (2011) JBC 286(9); 7190-7201).

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected USP46 in Human kidney tissue sections.

Immunoprecipitation Analysis: A representative lot detected USP46 in Immunoprecipitation applications (Joo, et al. (2011) JBC 286(9); 7190-7201).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected USP46 in Chromatin Immunoprecipitation applications (Joo, et al. (2011) JBC 286(9); 7190-7201).

Immunocytochemistry Analysis: A representative lot detected USP46 in Immunocytochemistry applications (Huo Y. (2015) J. Neurochem. 134(6); 1067-1080).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

Ubiquitin carboxyl-terminal hydrolase 46 (UniProt: P62068; also known as Deubiquitinating enzyme 46, Ubiquitin thioesterase 46, Ubiquitin-specific-processing protease 46, EC:3.4.19.12) is encoded by the USP46 gene (Gene ID: 64854) in human. USP46 gene encodes cysteine protease belonging to the ubiquitin-specific protease (USP) family of proteins. The protein contains a large USP domain (aa 35-365) and functions as a deubiquitinating enzyme capable of modifying cellular proteins. Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP46 cleaves ubiquitin from a ubiquitin-β-galactosidase fusion protein. These proteins regulate ubiquitin-mediated pathways that are involved in normal and pathological functions. It is also involved in the regulation of GABA action. Studies show that USP46, along with USP12, deubiquitinates both histone H2A and H2B. USP46 is predominantly localized in the cytoplasm. It is broadly expressed in multiple tissues, with high expression in heart muscle and CNS. Mutations or dysregulation of USP46 have been implicated in various diseases, particularly neuropsychiatric and neurological disorders. Additionally, USP46 may play a role in cancer, where its function in regulating protein stability and signaling pathways can contribute to tumor progression and resistance to therapy.(Ref.: Joo, et al. (2011). JBC 286(9); 7190-7201; Huo, Y., et al. (2015). J. Neurochem. 134(6); 1067-1080; Spencer, Z., et al. (2023). Nat Commun. 5;14(1); 6174).

Forme physique

Rabbit polyclonal antiserum without azide.

Reconstitution

Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable