ABS1051
Anti-Mitoferrin-1/Mfrn1 Antibody
serum, from rabbit
Synonyme(s) :
Mitoferrin-1, Mitoferrin-1/Mfrn1, Mitochondrial iron transporter 1, Mitochondrial solute carrier protein, Solute carrier family 25 member 37
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
serum
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
mouse
Technique(s)
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
mouse ... Slc25A37(67712)
Description générale
Mitoferrin-1 (UniProt Q920G8; also known as mitochondrial iron transporter 1, mitochondrial solute carrier protein, solute carrier family 25 member 37) is encoded by the Slc25a37 (also known as Mfrn, Mfrn1, Mscp) gene (Gene ID 67712) in murine species. Mitoferrin-1 and mitoferrin-2 (Slc25a28, Mfrn2) mediate iron import into mitochondria. Mitoferrin-2 is expressed ubiquitously, whereas mitoferrin-1 is specifically induced in differentiating erythroid cells. Mfrn1 knockout in zebrafish and mice disrupts heme and [Fe-S] cluster synthesis as a result of a severe reduction in mitochondrial iron in erythroid progenitors.
Spécificité
This antiserum does not react with spliced isofrms 2, 3, 4, and 5.
Immunogène
Epitope: C-terminus
Linear peptide corresponding to the C-terminus of mouse Mitoferrin-1/Mfrn1.
Application
Research Category
Signaling
Signaling
Research Sub Category
Developmental Signaling
Developmental Signaling
This Anti-Mitoferrin-1/Mfrn1 Antibody is validated for use in Western Blotting for the detection of Mitoferrin-1/Mfrn1.
Western Blotting Analysis: A 1:2,000 dilution of this antiserum detected mitoferrin-1 upregulation in mitochondria lysates from mouse erythroleukemia (MEL) cultures chemically induced to undergo erythroid maturation (Courtesy of Dr Barry H. Paw, Brigham and Women′s Hospital, Boston, MA).
Western Blotting Analysis: A representative lot detected reduced mitoferrin-1 expression due to heterozygous gene trap insertion in the Mfrn1 gene (Mfrn1+/gt) and loss of mitoferrin-1 expression due to homozygous Mfrn1 gene insertion (Mfrn1gt/gt) in mouse embryonic stem cells (mESCs) undergoing primitive erythropoiesis (Chung, J., et al.(2014). J Biol Chem. 289(11):7835-7843).
Western Blotting Analysis: A representative lot detected upregulated mitoferrin-1 expression following DMSO-induced erythroid maturation in mouse erythroleukemia (MEL) cultures (Shah, D.I., et al. (2012). Nature. 491(7425):608-612).
Western Blotting Analysis: A representative lot detected reduced mitoferrin-1 expression due to heterozygous gene trap insertion in the Mfrn1 gene (Mfrn1+/gt) and loss of mitoferrin-1 expression due to homozygous Mfrn1 gene insertion (Mfrn1gt/gt) in mouse embryonic stem cells (mESCs) undergoing primitive erythropoiesis (Chung, J., et al.(2014). J Biol Chem. 289(11):7835-7843).
Western Blotting Analysis: A representative lot detected upregulated mitoferrin-1 expression following DMSO-induced erythroid maturation in mouse erythroleukemia (MEL) cultures (Shah, D.I., et al. (2012). Nature. 491(7425):608-612).
Qualité
Evaluated by Western Blotting in mouse embryonic stem (mES) cell lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antiserum detected mitoferrin-1 in mouse embryonic stem (mES) cell lysates.
Western Blotting Analysis: A 1:5,000 dilution of this antiserum detected mitoferrin-1 in mouse embryonic stem (mES) cell lysates.
Description de la cible
~34 kDa observed. Uncharacterized band(s) may appear in some lysates.
Forme physique
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
Stockage et stabilité
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Mitochondrial Atpif1 regulates haem synthesis in developing erythroblasts.
Shah, DI; Takahashi-Makise, N; Cooney, JD; Li, L; Schultz, IJ; Pierce, EL; Narla et al.
Nature null
Jacky Chung et al.
The Journal of biological chemistry, 289(11), 7835-7843 (2014-02-11)
Mitochondrial iron is essential for the biosynthesis of heme and iron-sulfur ([Fe-S]) clusters in mammalian cells. In developing erythrocytes, iron is imported into the mitochondria by MFRN1 (mitoferrin-1, SLC25A37). Although loss of MFRN1 in zebrafish and mice leads to profound
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