ABS1026
Anti-Rab27B Antibody
serum, from rabbit
Synonyme(s) :
Ras-related protein Rab-27B, C25KG
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
serum
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
human, rat, mouse
Technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... RAB27B(5874)
Description générale
RAB27B, also known as C25KG, is a member of the Rab GTPase superfamily. RAB27B acts as a lipid anchoring protein that is prenylated and works in vesicular fusion and protein trafficking within cells. The protein is most highly expressed in testis but can be expressed in other cell types as well. Because Rab27B is widely expressed in various tissues together with Rab27A and RAB27B has been found to have the ability to bind all of the Rab27A effectors that have been tested, Rab27A and Rab27B were initially thought to function redundantly by sharing common Rab27 effectors. However, recent evidence has indicated that by interacting with different Rab27 effectors Rab27A and Rab27B play different roles in special types of secretion (e.g. exosome secretion and mast cell secretion) even within the same cell type. Rab27B is particularly important for regulating protein secretion by pancreatic acinar cells, for instance.
Immunogène
GST-tagged recombinant protein corresponding to human Rab27B.
Application
Research Category
Signaling
Signaling
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
This Anti-Rab27B Antibody is validated for use in Western Blotting and Immunoprecipitation and Immunohistochemistry for the detection of Rab27B.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Rab27B in 10 µg of C2C12, mouse brain, and PC3 lysate.
Western Blotting Analysis: A representative lot detected Rab27B in rat parotid gland lysate (Imai, A., et al. (2004). Journal of Cell Science. 117:1945-1953).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Rab27B in flg-Rab27B (Imai, A., et al. (2004). Journal of Cell Science. 117:1945-1953).
Immunohistochemistry Analysis: A representative lot detected Rab27B in rat parotid acinar cells (Imai, A., et al. (2004). Journal of Cell Science. 117:1945-1953).
Western Blotting Analysis: A representative lot detected Rab27B in rat parotid gland lysate (Imai, A., et al. (2004). Journal of Cell Science. 117:1945-1953).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Rab27B in flg-Rab27B (Imai, A., et al. (2004). Journal of Cell Science. 117:1945-1953).
Immunohistochemistry Analysis: A representative lot detected Rab27B in rat parotid acinar cells (Imai, A., et al. (2004). Journal of Cell Science. 117:1945-1953).
Qualité
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rab27B in 10 µg of mouse brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rab27B in 10 µg of mouse brain tissue lysate.
Description de la cible
~27 kDa observed. Uncharacterized band(s) may appear in some lysates.
Forme physique
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
Stockage et stabilité
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Certificats d'analyse (COA)
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Akane Imai et al.
Journal of cell science, 117(Pt 10), 1945-1953 (2004-03-25)
Small GTPase Rab is a large family of putative membrane trafficking proteins, and each member is thought to regulate a specific type(s) of membrane trafficking. However, little is known about the involvement of Rab protein(s) in secretory granule exocytosis in
Hiroyuki Nojima et al.
PloS one, 11(8), e0161443-e0161443 (2016-08-24)
Exosomes are small membrane vesicles released by different cell types, including hepatocytes, that play important roles in intercellular communication. We have previously demonstrated that hepatocyte-derived exosomes contain the synthetic machinery to form sphingosine-1-phosphate (S1P) in target hepatocytes resulting in proliferation
Rachel Underwood et al.
The Journal of biological chemistry, 295(23), 8005-8016 (2020-05-01)
α-Synuclein (αsyn) is the primary component of proteinaceous aggregates termed Lewy bodies that pathologically define synucleinopathies including Parkinson's disease (PD) and dementia with Lewy bodies (DLB). αsyn is hypothesized to spread through the brain in a prion-like fashion by misfolded
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