ABN2290

Anti-LHFPL5

• Synonyme(s) : LHFPL tetraspan subfamily member 5 protein, Lipoma HMGIC fusion partner-like 5 protein, Tetraspan membrane protein of hair cell stereocilia
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: N/A, polyclonal
• Poids mol.: calculated mol wt 24.2 kDa
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): monkey
• Conditionnement: antibody small pack of 100 μL
• Technique(s): immunocytochemistry: suitable; western blot: suitable
• Isotype: IgG
• Séquence de l'épitope: C-terminal
• Numéro d'accès Protein ID: NP_872354
• Numéro d'accès UniProt: Q8TAF8
• Température de stockage: -10 to -25°C
• Informations sur le gène: human ... LHFPL5(222662)

Description

Description générale

LHFPL tetraspan subfamily member 5 protein (UniProt: Q8TAF8; also known as Lipoma HMGIC fusion partner-like 5 protein, Tetraspan membrane protein of hair cell stereocilia) is encoded by the LHFPL5 (also known as TMHS) gene (Gene ID: 222662) in human. LHFPL5 is a multi-pass membrane protein with three cytoplasmic domains, four transmembrane domains, and two extracellular domains. Its localization to the plasma membrane requires the presence of protocadherin related 15 (PCDH15). LHFPL5 is recognized as a critical component of the mechano-transduction complex in inner-ear hair cells. It is shown to physically interact with and stabilize transmembrane channel-like protein 1 (TMC1). The C-terminal half of LHFPL5 is involved in this interaction. The N-terminal half is involved in its dimerization and interaction with PCDH15. Mutations in LHFPL5 gene are known to cause autosomal recessive deafness that is characterized by non-syndromic sensorineural hearing loss from damage to the neural receptors of the inner ear. Knockout of LHFPL5 in murine models is shown to result in TMC1 reduction in the hair bundles of auditory cells. (Ref.: Yu, X., et al. (2020). Proc, Natl. Acad. Sci. USA. 117(47); 29894-29903).

Spécificité

This rabbit polyclonal antibody detects LHFPL tetraspan subfamily member 5 protein (LHFPL5). It targets an epitope within 20 amino acids from the C-terminal region.

Immunogène

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human LHFPL tetraspan subfamily member 5 protein (LHFPL5).

Application

Quality Control Applications

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected LHFPL5 in HeLa cells.

Tested applications

Western Blotting Analysis: A representative lot detected LHFPL5 in Western Blotting applications (Yu, Z., et. al. (2020). Proc Natl Acad Sci USA. 117(47); 29894-29903).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LHFPL5 in HEK293 cells transfected with LHFPL5 (TMHS) protein.

Immunocytochemistry Analysis: A representative lot detected LHFPL5 in Immunocytochemistry applications (Yu, Z., et. al. (2020). Proc Natl Acad Sci USA. 117(47); 29894-29903).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Forme physique

Rabbit polyclonal antibody in 0.02 mg/mL sodium azide.

Stockage et stabilité

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1