ABE496

Anti-Lysine-specific demethylase 5C Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Lysine-specific demethylase 5C, Histone demethylase JARID1C, Jumonji/ARID domain-containing protein 1C, Protein SmcX, Protein Xe169
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Technique(s): immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Numéro d'accès NCBI: NP_004178.2
• Numéro d'accès UniProt: P41229
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... KDM5C(8242)

Description

Description générale

Lysine-specific demethylase 5C is also called Histone demethylase JARID1C, Jumonji/ARID domain-containing protein 1C, Protein SmcX and Protein Xe169. Lysine-specific demethylase 5C is a histone demethylase that specifically targets trimethylated and dimethylated, but not monomethylated, ′Lys-4′ of histone H3, making it a regulator of the histone code. Lysine-specific demethylase 5C is involved in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements and is associated with Mental retardation, X-linked, syndromic, Claes-Jensen type (MRXSCJ). Lysine-specific demethylase 5C is widely expressed with highest levels found in brain and skeletal muscle.

Spécificité

This antibody is expected to react with all four known isoforms based upon immunogen design.

Immunogène

KLH-conjugated linear peptide corresponding to a region near the near the N-terminus of human Lysine-specific demethylase 5C.

Application

Detect JARID using this rabbit polyclonal antibody, Anti-Lysine-specific demethylase 5C Antibody validated for use in western blotting, IP & IHC.

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated Lysine-specific demethylase 5C in 10 µg of human hippocampus tissue lysate.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Lysine-specific demethylase 5C in human stomach tissue.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Qualité

Evaluated by Western Blotting in human hippocampus tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Lysine-specific demethylase 5C in 10 µg of human hippocampus tissue lysate.

Description de la cible

~170 kDa observed. Uniprot describes four isoforms at 176 kDa (isoform 1), 175 kDa (isoform 2), 171 kDa (isoform 3), and 155 kDa (isoform 4). Uncharacterized band(s) may be observed in some cell lysates.

Forme physique

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable