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Principaux documents

ABE26

Sigma-Aldrich

Anti-acetyl-c-Myc (Lys323) Antibody

from rabbit, purified by affinity chromatography

Synonyme(s) :

Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, HLHe39, Proto-oncogene c-Myc, Transcription factor p64, c-MYC

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

mouse, human

Réactivité de l'espèce (prédite par homologie)

rhesus macaque (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)

Technique(s)

western blot: suitable

Numéro d'accès NCBI

NP_002458

Numéro d'accès UniProt

P01106

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

acetylation (Lys323)

Informations sur le gène

chimpanzee ... Myc(464393)
human ... MYC(4609)
mouse ... Myc(17869)
rhesus monkey ... Myc(694626)

Description générale

c-Myc (bHLHe39) is a transcription factor that regulates the expression of multiple genes involved in apoptosis, cell growth, differentiation, and proliferation. Active c-Myc is coupled to the MAX protein via c-Myc’s C-terminal helix-loop-helix leucine zipper (bHLHLZ) domain. The c-Myc-MAX heterodimer also interacts with a number of other transcription-related proteins including TRRAP, p107 and Miz-1, and c-Myc-MAX may indirectly regulate histone acetylases involved in chromatin remodeling. c-Myc is itself acetylated on lysine 323 by GCN5, and on lysine 148 and 157 by p300/CBP. The significance of c-Myc acetylation is still under investigation; however, previous studies have suggested that HAT-dependent acetylation of c-Myc may regulate its turnover and stability. c-Myc may play a role in various cancers such as Burkitt’s lymphoma.

Spécificité

Other homologies: Rat, Feline, Sheep, and Bovine (90% sequence homology).
This antibody recognizes c-Myc acetylated at Lys323.

Immunogène

Epitope: Acetylated Lys323
KLH-conjugated linear peptide corresponding to human c-Myc acetylated at Lys323.

Application

Peptide Inhibition Assay Analysis: 2 µg/mL from a representative lot blocked acetyl-c-Myc (Lys323) in HEK293T co-transfected with wild type c-Myc or mutant c-Myc (Lys323R) and P300.

Peptide Inhibition Assay Analysis: A representative lot was used by an independent laboratory in HEK293T cells co-transfected with mouse wildtype c-Myc and P300. (Image courtesy of Dr. Ernest Martinez, Department of Biochemistry, University of California at Riverside.)
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Use Anti-acetyl-c-Myc (Lys323) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect acetyl-c-Myc (Lys323) also known as HLHe39, Proto-oncogene c-Myc, Transcription factor p64, c-MYC.

Qualité

Evaluated by Western Blot in transfected HEK293T cell lysates.

Western Blot Analysis: 0.2 µg/mL of this antibody detected c-Myc on 10 µg of transfected HEK293T cell lysates.

Description de la cible

~58 kDa observed. Uniprot describes two isoforms at 49 and 51 kDa

Forme physique

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Transfected HEK293T cell lysates

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Wesley W Wang et al.
Journal of the American Chemical Society, 143(40), 16700-16708 (2021-10-01)
Protein acetylation is a central event in orchestrating diverse cellular processes. However, current strategies to investigate protein acetylation in cells are often nonspecific or lack temporal and magnitude control. Here, we developed an acetylation tagging system, AceTAG, to induce acetylation
J T Lynch et al.
Cell death & disease, 4, e573-e573 (2013-04-06)
Using a screening strategy, we identified the tetratricopeptide repeat (TPR) motif protein, Tetratricopeptide repeat domain 5 (TTC5, also known as stress responsive activator of p300 or Strap) as required for the survival of human acute myeloid leukemia (AML) cells. TTC5

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