ABE1974

Anti-CHMP1B

from rabbit, purified by affinity chromatography

• Synonyme(s) : Charged multivesicular body protein 1b, CHMP1.5, Chromatin-modifying protein 1b, Vacuolar protein sorting-associated protein 46-2, Vps46-2, hVps46-2
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): mouse (based on 98% homology), bovine (based on 99% homology)
• Technique(s): western blot: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NP_065145
• Numéro d'accès UniProt: Q7LBR1
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CHMP1B(57132)

Description

Description générale

Charged multivesicular body protein 1b (UniProt: Q7LBR1; CHMP1.5, Chromatin-modifying protein 1b, CHMP1b, Vacuolar protein sorting-associated protein 46-2, Vps46-2, hVps46-2) is encoded by the CHMP1B (also known as C18orf2) gene (Gene ID: 57132) in human. CHMP1b is a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. It is widely expressed and is found in pancreas, kidney, skeletal muscle, liver, lung, placenta and the brain tissue. CHMP1b is a peripherally associated component of the endosomal sorting system that is required for transport complex III (ESCRT-III), which is involved in multi-vesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. CHMP1b is localized in cytosol, endosome, and in peripheral membrane protein. CHMP1b binds specifically to the AAA ATPase spastin and thereby recruits this microtubule severing protein to the midbody. CHMP1b and spastin are shown to colocalize at midbodies in dividing HeLa cells, and the knockdown of CHMP1b reduces the amount of spastin at midbodies.

Spécificité

This polyclonal antibody detects Charged multivesicular body protein 1b in human cells. Human CHMP1B displays 99% and 98% homology with bovine and mouse CHMP1B, respectively.

Immunogène

Epitope: unknown

GST-tagged full length recombinant human Charged multivesicular body protein 1b.

Application

Anti-CHMP1B, Cat. No. ABE1974, is a highly specific rabbit polyclonal antibody that targets charged multivesicular body protein 1b and has been tested in Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: A representative lot detected CHMP1B in Western Blotting applications (Morita, E., et. al. (2010). Proc Natl Acad. Sci USA. 107(29):12889-94; Bailey, J.K., et. al. (2015). J Biol Chem. 290(14):8987-9001).

Qualité

Evaluated by Western Blotting in ARPE-19 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected CHMP1B in 10 µg of ARPE-19 cell lysate.

Description de la cible

~29 kDa observed; 22.11 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1