ABE1057

Anti-acetyl Histone H3 Antibody (Lys 64)

from rabbit, purified by affinity chromatography

• Synonyme(s) : Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/I, Histone H3/j, Histone H3/k, Histone H3/l
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human, mouse
• Technique(s): dot blot: suitable; immunocytochemistry: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_003520
• Numéro d'accès UniProt: P68431
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: acetylation (Lys 64)
• Informations sur le gène: human ... HIST1H3F(8968)

Description

Description générale

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci. Histone H3 is reported to be acetylated at Lys64 (H3K64ac) by p300 co-activator. In addition, H3K64ac is found enriched at the transcriptional start sites of active genes, consistent with a transcriptional activation modification opposite of its repressive counterpart H3K64me3 (PMID 24668167). The target specificity of ABE1057 is confirmed with peptide-based epitope analysis by dot blot, using Cat. No. 16-667, AbSurance Histone H3 Antibody Specificity Array, and Cat. No. 16-665, AbSurance Histone H2A, H2B, H4 Antibody Specificity Array. Sequence surrounding the target acetylation site is conserved among human Histone H3 subtypes H3.1, H3.2, H3.3, and H3.3C/H3.5 (UniProt P68431, Q71DI3, P84243, and Q6NXT2, respectively).

Immunogène

Epitope: Acetylated Lys 64

KLH-conjugated linear peptide corresponding to human acetyl Histone H3 (Lys 64).

Application

Anti-acetyl Histone H3 Antibody is an antibody against acetyl Histone H3 for use in Western Blotting, Immunocytochemistry, Dot Blot.

Immunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected acetyl Histone H3 (Lys 64) in HeLa, A431, and NIH/3T3 cells.
Dot Blot Specificity Analysis: Histone peptides with various modifications were probed with acetyl Histone H3 (Lys 64) using a 1:250 dilution.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Qualité

Evaluated by Western Blotting in sodium butyrate treated HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected acetyl Histone H3 (Lys 64) in 10 µg of sodium butyrate treated HeLa cell lysate.

Description de la cible

~17 kDa observed

Forme physique

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable