ABE1032

Anti-ERp57 Antibody

serum, from rabbit

• Synonyme(s) : Protein disulfide-isomerase A3, 58 kDa glucose-regulated protein, 58 kDa microsomal protein, p58, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, ER protein 57, ERp57, Endoplasmic reticulum resident protein 60, ER protein 60, ERp60
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: serum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human, rat, mouse
• Technique(s): ChIP: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_005304
• Numéro d'accès UniProt: P30101
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PDIA3(2923)

Description

Description générale

Endoplasmic reticulum resident protein 57 (ERp57) is also called Protein disulfide-isomerase A3 (PDIA3), 58 kDa glucose-regulated protein (GRP58), 58 kDa microsomal protein (p58), Disulfide isomerase ER-60, and Endoplasmic reticulum resident protein 60 (ERp60). ERp57 catalyzes the rearrangement of -S-S- bonds in proteins and is associated with signal transduction, nuclear protein import and apoptosis. ERp57 is expressed in the flagellum and head regions of spermatozoa.

Immunogène

Recombinant protein corresponding to human ERp57.

Application

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory lot detected ERp57 in M14 cell lysates (Chichiarelli, S., et al. (2010). Arch Biochem Biophys. 494(2):178-183; Chichiarelli, S., et al. (2007). J Cell Physiol. 210(2):343-351.).

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected ERp57 in NPA, WRO, ARO and NCI cell lysates (Prof. F. Altieri, University of Rome, La Sapienza).

Detect ERp57 using this rabbit polyclonal antibody, Anti-ERp57 Antibody validated for use in western blotting & Chromatin IP (ChIP).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Qualité

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1:1,000 dilution of this antibody detected ERp57 in 10 µg of HeLa cell lysate.

Description de la cible

~57 kDa observed

Forme physique

Rabbit polyclonal in buffer containing serum with 0.05% sodium azide.

Unpurified

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1