ABD99

Anti-Rex-1 Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Zinc finger protein 42, Reduced expression protein 1, mREX-1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Technique(s): immunocytochemistry: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_777560
• Numéro d'accès UniProt: Q96MM3
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ZFP42(132625)

Description

Description générale

The Rex-1 (Zfp-42) gene, which encodes an acidic zinc finger protein, is expressed at high levels in embryonic stem (ES) and F9 teratocarcinoma cells. The Rex-1 promoter is regulated by specific octamer family members in early embryonic cells, and is involved in retinoic acid (RA)-associated reduction in expression. Rex-1 is Involved in self-renewal property of ES cells and may be involved in transcriptional regulation of pluripotency signaling pathways. Rex-1 is also involved in the reprogramming of X-chromosome inactivation during the acquisition of pluripotency and is required for efficient elongation of TSIX, a non-coding RNA antisense to XIST.

Immunogène

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of Human Rex-1.

Application

Detect Rex-1 using this Anti-Rex-1 Antibody validated for use in western blotting & ICC.

Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Rex-1 in a undifferentiated H9 human embryonic stem cells.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

Qualité

Evaluated by Western Blotting in a undifferentiated H9 human embryonic stem cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected Rex-1 in undifferentiated H9 human embryonic stem cells.

Description de la cible

~35 kDa observed. Uncharacterized band(s) may appear in some lysates.

Forme physique

Antigen Affinity Purified

Purified rabbit Polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Undifferentiated H9 human embryonic stem cells.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable