ABC1451

Anti-PDGF-D

from rabbit

• Synonyme(s) : Platelet-derived growth factor D, Iris-expressed growth factor, Spinal cord-derived growth factor B, SCDGF-B
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): rabbit (based on 100% sequence homology)
• Conditionnement: antibody small pack of 25 μg
• Technique(s): western blot: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NP_079484
• Numéro d'accès UniProt: Q9GZP0
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PDGFD(80310)

Description

Description générale

Platelet-derived growth factor D (UniProt: Q9GZP0; also known as PDGF-D, Iris-expressed growth factor, Spinal cord-derived growth factor B, SCDGF-B) is encoded by the PDGFD (also known as IEGF, SCDGFB, MSTP036, UNQ1899/PRO4345) gene (Gene ID: 80310) in human. Four PDGF ligands known as PDGF-A, PDGF-B, PDGF-C, and PDGF-D have been described that exert their effect by binding to their cognate receptors. PDGF-D is expressed at high levels in the heart, pancreas, adrenal gland and ovary and at low levels in placenta, liver, kidney, prostate, testis, small intestine, spleen, and colon. PDGF-D is synthesized with a signal peptide (aa 1-18), which is subsequently removed. The latent form of PDGF-D contains a CUB domain at its N-terminal region, a growth factor domain and a hinge region in between. The latent form is activated by the proteolytic action of matriptase that removes the CUB domain, which results in unmasking of the receptor-binding epitopes on the core domain. YR247GR249S within the PDGF-D hinge region is shown to be the cleavage site for removal of the inhibitory CUB domain. PDGF-D plays an important role in wound healing and induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. PDGF-D also induces cellular transformation and promotes tumor growth by accelerating the proliferation rate of tumor cells and by stimulation of tumor neovascularization. (Ref.: Huang, W., and Kim, H-R, C (2015). J. Biol. Chem. 290(14); 9162-9170).

Spécificité

This rabbit polyclonal antibody detects Platelet-derived growth factor D in human cells. It targets an epitope within the growth factor domain from the C-terminal half.

Immunogène

A linear peptide corresponding to 18 amino acids from the C-terminal half of human Platelet-derived growth factor D.

Application

Anti-PDGF-D, Cat. No. ABC1451, is a rabbit polyclonal antibody that detects Platelet-derived growth factor D in human cells and has been tested for use in Western Blotting.

Western Blotting Analysis: A representative lot detected PDGF-D in human recombinant-PDGF D (Huang, W., et. al. (2015). J Biol Chem. 290(14):9162-70) and LNCaP cells expressing PDGF-D, BS-C-1 cells transfected with the plasmid pTF7-PDGF D:HIS, recombinant PDGF-D (Ustach, C.V., et. al. (2004). Cancer Res. 64(5):1722-9).

Western Blotting Analysis: A representative lot detected PDGF-D in PBS exchanged condition media collected from LNCaP cells transfected with PDGF-D expression vector (Courtesy of Hyeong-Reh Choi Kim lab at Wayne State University).

Qualité

Evaluated by Western Blotting condition media collected from LNCaP cells transfected with PDGF-D expression vector.

Western Blotting Analysis: 2 µg/mL of this antibody detected PDGF-D in PBS exchanged condition media collected from LNCaP cells transfected with PDGF-D expression vector.

Description de la cible

~90 kDa Full Length PDGF D dimer, ~52 kDa PDGF D hemi-dimer,~32 kDa growth factor dimer in the non-reducing conditions; 42.85 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Purified rabbit polyclonal antibody in PBS (pH 7.4) with 0.09% sodium azide.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2