AB951-I

Anti-Neuron Specific Enolase Antibody

from rabbit

• Synonyme(s) : Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human, mouse, rat
• Réactivité de l'espèce (prédite par homologie): bovine (based on 100% sequence homology)
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Numéro d'accès NCBI: NP_001966
• Numéro d'accès UniProt: P09104
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ENO2(2026)

Description

Description générale

The glycolytic enzyme enolase (2-phospho-D-glycerate hydrolase, E.C. 4.2.1.11) is composed of two subunits and exists in three molecular forms in human brain: non-neuronal enolase (NNE; alpha, alpha′-enolase), neuron-specific enolase (NSE; gamma,gamma′-enolase, also known as 14-3-2 protein and NSP) and the short-lived heterodimer alpha, gamma′-enolase. The neuron specific form is a highly acidic homodimeric protin of 78kDa, which has been found in central and peripheral nervous elements and also in many neuroendocrine cells and derivative tumors. NSE is regarded as the best described serum marker for small cell lung cancer. In addition, the measurement of NSE levels in both serum and cerebrospinal fluid following cerebral ischaemia and traumatic head injury provides a reliable laboratory indicator of the degree of brain cell damage and may allow early prediction of prognostic outcome.

Spécificité

This antibody recognizes a region near the N-terminus of Neuron Specific Enolase.

Immunogène

Epitope: Near N-terminus

KLH-conjugated linear peptide corresponding to the near N-terminus of human Neuron Specific Enolase.

Application

Anti-Neuron Specific Enolase Antibody is a highly specific rabbit polyclonal antibody, that targets Neuron-Specific Enolase & has been tested in western blotting & IHC (Paraffin).

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected in Neuron Specific Enolase in human thalamus tissue and glioma sections.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Qualité

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Neuron Specific Enolase in 10 µg of rat brain tissue lysate.

Description de la cible

~47 kDa observed. Cat. No. AB951, Anti-Neuron Specific Enolase detects Alpha Enolase (ENO1) at ~39kDa However, Neuron Specific Enolase has been observed at ~48 kDa (Braus, B., K., et al (2008). Vet Immunol Immunopathol 124(1-2):177-183.; Feo, S., et al. (2000). FEBS Lett. 473(1):47-52.).

Liaison

Replaces: AB951

Forme physique

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Rat brain tissue lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable