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Principaux documents

AB5818

Sigma-Aldrich

Anti-Potassium Channel Kir4.1 Antibody

Chemicon®, from rabbit

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Forme d'anticorps

affinity purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

rat, mouse, human

Fabricant/nom de marque

Chemicon®

Technique(s)

western blot: suitable

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... KCNJ10(3766)

Spécificité

Recognizes Kir4.1. The epitope does not share homology with any other known proteins.

SPECIES REACTIVITIES: The antibody is also expected to work with mouse and human due to sequence homology. Other species have not been tested.

Immunogène

CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user. Synthetic peptide corresponding to amino acids 356-375 of rat, mouse or human Kir4.1 (Accession P49655).

Application

This Anti-Potassium Channel Kir4.1 Antibody is validated for use in WB for the detection of Potassium Channel Kir4.1.
Western blot: 1:200-1:600 using ECL on rat brain membranes or human platelets.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Forme physique

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for

Remarque sur l'analyse

Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Mentions de danger

Conseils de prudence

Classification des risques

Aquatic Chronic 3

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Zuzanna Setkowicz et al.
Journal of anatomy, 231(3), 366-379 (2017-06-10)
Astrocytes participate in neuronal development and excitability, and produce factors enhancing or suppressing inflammatory processes occurring due to neurodegenerative diseases, such as epilepsy. Seizures, in turn, trigger the release of inflammatory mediators, causing structural and functional changes in the brain.
Meritxell Espino Guarch et al.
eLife, 7 (2018-01-23)
Age-related hearing loss (ARHL) is the most common sensory deficit in the elderly. The disease has a multifactorial etiology with both environmental and genetic factors involved being largely unknown. SLC7A8/SLC3A2 heterodimer is a neutral amino acid exchanger. Here, we demonstrated
Yue Yang et al.
PloS one, 6(1), e16547-e16547 (2011-02-10)
The endothelial-blood/tissue barrier is critical for maintaining tissue homeostasis. The ear harbors a unique endothelial-blood/tissue barrier which we term "blood-labyrinth-barrier". This barrier is critical for maintaining inner ear homeostasis. Disruption of the blood-labyrinth-barrier is closely associated with a number of
Steven A Hill et al.
eLife, 8 (2019-06-14)
Astrocytes have emerged as integral partners with neurons in regulating synapse formation and function, but the mechanisms that mediate these interactions are not well understood. Here, we show that Sonic hedgehog (Shh) signaling in mature astrocytes is required for establishing

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