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Key Documents

AB5574

Sigma-Aldrich

Anti-TrpC6 Antibody

Chemicon®, from rabbit

Synonyme(s) :

Transient Receptor Potential-Like Channel 6, Trp6

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

affinity purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

rat

Fabricant/nom de marque

Chemicon®

Technique(s)

immunohistochemistry: suitable
western blot: suitable

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

Spécificité

Recognizes TRPC6 (Transient Receptor Potential-Like Channel 6, TRP-6). Does not cross react with any other known proteins.

SPECIES REACTIVITIES: It is expected that the antibody will also work on human due to sequence conservation (15/16). Other species have not been tested.

Immunogène

Peptide corresponding to amino acids 24-38 from mouse TRPC6 (Zhu et al. 1996). Accession Q61143

Application

Anti-TrpC6 Antibody is an antibody against TrpC6 for use in IH & WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Western blot:1:200-1:400 using ECL on rat brain membranes.
Immunohistochemstry on mouse brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%)
Addition of 0.1-0.5% Tween-20 to the antibody solution is recommended.
Optimal working dilutions must be determined by the end user.

Forme physique

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Stockage et stabilité

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Remarque sur l'analyse

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Mentions de danger

Conseils de prudence

Classification des risques

Aquatic Chronic 3

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Ji-Eun Kim et al.
Frontiers in cellular neuroscience, 11, 413-413 (2018-01-13)
Recently, we have reported that transient receptor potential channel-6 (TRPC6) plays an important role in the regulation of neuronal excitability and synchronization of spiking activity in the dentate granule cells (DGC). However, the underlying mechanisms of TRPC6 in these phenomena

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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