AB3573
Anti-BCL-XL (pS62) Antibody
Chemicon®, from rabbit
Synonyme(s) :
Bcl-2-binding component 6, Bcl-XL/Bcl-2-associated death promoter, Bcl-2-like 8 protein, BAD, BBC6, BCL2L8
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
human
Réactivité de l'espèce (prédite par homologie)
rat (100% homologous), mouse (100% homologous)
Fabricant/nom de marque
Chemicon®
Technique(s)
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
phosphorylation (pSer62)
Informations sur le gène
human ... BCL2L1(598)
Description générale
Bcl-xL is a ~28 kDa member of Bcl-2 family of proteins and an important regulator of apoptosis. Bcl-xL forms heterodimers with BAX, BAK, and Bcl-2, and its overexpression in tumor cells confers resistance against chemotherapeutic drugs. Bcl-xL is phosphorylated on many sites including serine 62, a critical site for Bcl-xL response to microtubule-damaging drugs such as taxol and vinblastine. Phosphorylation of serine 62 (thought to be mediated by Jun N-terminal stress kinase (JNK) signaling) negatively regulates the anti-apoptotic function of Bcl-xL and controls the growth of neoplastic cells.
Spécificité
Human Bcl-xL. Mouse and rat Bcl-xL (100% conserved) have not been tested, but are expected to react.
Immunogène
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Bcl-xL that contains serine 62. The sequence is conserved in mouse and rat.
Application
Anti-BCL-XL (pS62) Antibody detects level of BCL-XL (pS62) & has been published & validated for use in WB.
For Western blotting applications, we recommend using the antibody at a 1:1000 dilution.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
BCL2 & Inhibition
BCL2 & Inhibition
Qualité
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Bcl-xL. The final product is generated by affinity chromatography using a Bcl-xL-derived peptide that is phosphorylated at serine 62.
Liaison
Replaces: 04-432
Forme physique
Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide.
Stockage et stabilité
Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.
Remarque sur l'analyse
Control
SK-BR-3 cells treated with vinblastine.
SK-BR-3 cells treated with vinblastine.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Anti-HLA class I antibody-mediated activation of the PI3K/Akt signaling pathway and induction of Bcl-2 and Bcl-xL expression in endothelial cells.
Jin, Y.P., et al.
Human immunology, 65(4), 291-302 (2004)
Ochratoxin A induces apoptosis in human lymphocytes through down regulation of Bcl-xL.
Assaf, Hind, et al.
Toxicological Sciences, 79, 335-344 (2004)
Selective induction of apoptosis by PBOX-6 in leukemia cells occurs via the JNK dependent phosphorylation and inactivation of Bcl-2 and Bcl-XL.
Mc Gee, M.M., et al.
Journal of Pharmacology and Experimental Therapeutics, 310(3), 1084-1095 (2004)
Bcl-2 and Bcl-XL with nonpeptidic small-molecule antagonists.
Wang, S., et al.
Seminars in Oncology, 30(5 Suppl 16), 133-142 (2003)
K Sugiyama et al.
Cancer research, 59(17), 4406-4412 (1999-09-15)
7-Hydroxystaurosporine (UCN-01) is a protein kinase inhibitor that is under development as an anticancer agent in the United States and Japan. Long-term exposure of human A549 non-small cell lung cancer cells to UCN-01 furnished cells (A549/UCN) with acquired resistance against
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