AB2215
Anti-Interferon-β Antibody
serum, Chemicon®
Synonyme(s) :
Anti-IFB, Anti-IFF, Anti-IFN-beta, Anti-IFNB
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
serum
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
mouse
Fabricant/nom de marque
Chemicon®
Technique(s)
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
neutralization: suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
mouse ... Ifnb1(15977)
Spécificité
Reacts with mouse Interferon beta (IFNbeta). No reactivity is seen with IFNalpha or gamma
Immunogène
Mouse IgG, whole molecule.
Application
Cytopathic effect inhibition assay: 2 x 10E5 neutralization units per mL of antiserum. One neutralization unit is the amount of antiserum required to neutralize 1 unit of mouse IFN-beta. This material is prepared specifically for effective neutralization of mouse IFN-beta.
Western blot (1:500) The antibody reacts with a 19kDa band coresponding to Mu-IFN-beta. Protocol below:
1. Put protein (approx 50ng) in blocking solution for 30-60 min RT
2. Add Primary antibody (1:500) to blocking solution
3. Agitate membrane for 60 min, RT
4. Wash 3X for 5min each with PBS-Tween
5. Add secondary antibody (1:1000) after diluting in PBS-Tween
6. Gentle agitation for 60min.
7. Wash 3X for 5min each with PBS-Tween
8. Develop with reaction kit of choice
Immunoprecipitation
Immunohistochemistry
ELISA
Optimal working dilutions must be determined by end user.
Western blot (1:500) The antibody reacts with a 19kDa band coresponding to Mu-IFN-beta. Protocol below:
1. Put protein (approx 50ng) in blocking solution for 30-60 min RT
2. Add Primary antibody (1:500) to blocking solution
3. Agitate membrane for 60 min, RT
4. Wash 3X for 5min each with PBS-Tween
5. Add secondary antibody (1:1000) after diluting in PBS-Tween
6. Gentle agitation for 60min.
7. Wash 3X for 5min each with PBS-Tween
8. Develop with reaction kit of choice
Immunoprecipitation
Immunohistochemistry
ELISA
Optimal working dilutions must be determined by end user.
Detect Interferon-β using this Anti-Interferon-β Antibody validated for use in ELISA, IP, WB, IH, NEUT.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
Cytokines & Cytokine Receptors
Conditionnement
2x10(e4) units
Forme physique
Rabbit polyclonal serum in buffer containing 0.3 M NaHCO3 and 200 mM NaCl.
Stockage et stabilité
Maintain frozen at -20°C or below for retention of full activity, for up to 12 months. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Choon Kit Tang et al.
PloS one, 8(3), e60038-e60038 (2013-04-05)
5,6-Dimethylxanthenone-4-acetic acid (DMXAA), a potent type I interferon (IFN) inducer, was evaluated as a chemotherapeutic agent in mouse cancer models and proved to be well tolerated in human cancer clinical trials. Despite its multiple biological functions, DMXAA has not been
Dwijit GuhaSarkar et al.
Molecular oncology, 11(2), 180-193 (2017-01-18)
The highly invasive property of glioblastoma (GBM) cells and genetic heterogeneity are largely responsible for tumor recurrence after the current standard-of-care treatment and thus a direct cause of death. Previously, we have shown that intracranial interferon-beta (IFN-β) gene therapy by
A convenient and rapid cytopathic effect inhibition assay for interferon.
P C Familletti et al.
Methods in enzymology, 78(Pt A), 387-394 (1981-01-01)
S Rubinstein et al.
Journal of virology, 37(2), 755-758 (1981-02-01)
A convenient assay for interferons based on reduction of cytopathic effect was developed. The number of manipulations and the lengths of the various incubation steps were reduced to a minimum. The assay is simple to perform and can be completed
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