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Key Documents

AB2034

Sigma-Aldrich

Anti-Laminin Antibody

Chemicon®, from rabbit

Synonyme(s) :

Anti-LAMA, Anti-PTBHS, Anti-S-LAM-alpha

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

affinity purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

mouse

Fabricant/nom de marque

Chemicon®

Technique(s)

ELISA: suitable
immunohistochemistry: suitable
radioimmunoassay: suitable

Numéro d'accès NCBI

NM_005559.2

Numéro d'accès UniProt

P25391

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

mouse ... Lama1(16772)

Spécificité

Laminin extracted and purified from mouse tumor tissues. Antibody shows less than 0.1% reactivity with mouse collagen Types I, II, and IV; rat collagen Types I, III, and V; rat and human elastin; mouse fibronectin and laminin by radioimmunoassay.

Immunogène

Laminin extracted and purified from EHC mouse tumor tissues.

Application

Anti-Laminin Antibody detects level of Laminin & has been published & validated for use in ELISA, RIA, IH.
IFA (with fluorescein anti-rabbit IgG conjugate) - use diluted at ≥ 1:80 on frozen mouse tissues (skin, liver, etc.).
IHC (paraffin) - use diluted at ≥ 1:500 on fixed, paraffin-embedded mouse tissues (skin, liver, etc). (immunoperoxidase technique with the Envision / Dako kit);
ELISA - use diluted at ≥ 1:200 (OD ≥ 500).
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Forme physique

The purified antibody is supplied in a buffer containing a mixture of 0.1M Citrate, 0.1M potassium phosphate, at a pH of 7.2-7.4 and 10µl/ml of antibiotics and antimycotics.

Stockage et stabilité

Maintain frozen at -20°C for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Wnt signaling mediates self-organization and axis formation in embryoid bodies.
ten Berge, D; Koole, W; Fuerer, C; Fish, M; Eroglu, E; Nusse, R
Cell Stem Cell null
Dual embryonic origin of the mammalian otic vesicle forming the inner ear.
Freyer, L; Aggarwal, V; Morrow, BE
Development null
Upregulation of transforming growth factor-beta1 and vascular endothelial growth factor gene and protein expression in cyclosporin-induced overgrown edentulous gingiva in rats.
Yen-Teen Chen, Hsiao-Pei Tu, Yu-Tang Chin, E-Chin Shen, Cheng-Yang Chiang, Ching-Hwa Gau, Earl Fu
Journal of Periodontology null
Pascal Stuelsatz et al.
Developmental biology, 385(2), 366-379 (2013-08-24)
Extraocular muscles (EOM) represent a unique muscle group that controls eye movements and originates from head mesoderm, while the more typically studied body and limb muscles are somite-derived. Aiming to investigate myogenic progenitors (satellite cells) in EOM versus limb and
Fas-mediated upregulation of vascular endothelial growth factor and monocyte chemoattractant protein-1 expression in cultured dermal fibroblasts: role in the inflammatory response.
Masao Fujiwara, Hiroki Suemoto, Yasuteru Muragaki, Akira Ooshima
The Journal of Dermatology null
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