AB1534
Anti-Brain Derived Neurotrophic Factor Antibody
serum, Chemicon®
Synonyme(s) :
brain-derived neurotrophic factor
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
serum
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
rat, human, mouse
Fabricant/nom de marque
Chemicon®
Technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
Adéquation
not suitable for activity/function inhibition
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... BDNF(627)
Description générale
Brain Derived Neurotrophic Factor (BDNF) is a secreted protein that promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer’s and Huntington disease patients. BDNF may play a role in the regulation of stress response and in the biology of mood disorders. BDNF exists as monomers or homodimers and binds to TRKB/NTRK2 receptors.
Spécificité
Brain Derived Neurotrophic Factor (BDNF). By dot blot the antibody shows less than 0.1% cross reactivity with recombinant human NT3, NT4/5 or mouse NGF.
Immunogène
A 10 amino acid peptide corresponding to the amino-terminal of mouse Brain Derived Neurotrophic Factor (BDNF) coupled to ovalbumin.
Application
Immunohistochemistry:
1:1000-1:2000 dilution of a previous lot was used in immunohistochemistry (see suggested protocol).
ELISA:
1:1000-1:2000 dilution of a previous lot was used in ELISA.
Bioassay:
Does not inhibit BDNF biological activity.
Optimal working dilutions must be determined by end user.
1:1000-1:2000 dilution of a previous lot was used in immunohistochemistry (see suggested protocol).
ELISA:
1:1000-1:2000 dilution of a previous lot was used in ELISA.
Bioassay:
Does not inhibit BDNF biological activity.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
Neuroinflammation & Pain
Neurochemistry & Neurotrophins
Neuroinflammation & Pain
This Anti-Brain Derived Neurotrophic Factor Antibody is validated for use in ELISA, IH, WB for the detection of Brain Derived Neurotrophic Factor.
Qualité
Evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected BDNF on 10 µg of mouse brain lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected BDNF on 10 µg of mouse brain lysates.
Description de la cible
18 kDa, with a homodimer at ~27-30 kDa
Forme physique
Rabbit polyclonal antiserum in buffer containing no preservatives. Lyophilized. Reconstitute with 50 μL of sterile distilled water.
Unpurified
Stockage et stabilité
Maintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Remarque sur l'analyse
Control
Brain tissue
Brain tissue
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Role of brain-derived neurotrophic factor in wobbler mouse motor neuron disease.
K Tsuzaka, T Ishiyama, E P Pioro, H Mitsumoto, K Tsuzaka, T Ishiyama, E P Pioro, H Mitsumoto
Muscle and Nerve null
Neuronal loss and expression of neurotrophic factors in a model of rat chronic compressive spinal cord injury.
Kazuma Kasahara, Takao Nakagawa, Toshihiko Kubota, Kazuma Kasahara, Takao Nakagawa, Toshihiko Kubota
Spine null
Qualitative analysis of hippocampal plastic changes in rats with epilepsy supplemented with oral omega-3 fatty acids.
Roberta M Cysneiros,Danuza Ferrari,Ricardo M Arida,Vera C Terra et al.
Epilepsy & Behavior : E&B null
Levels of brain-derived neurotrophic factor and neurotrophin-4 in lumbar motoneurons after low-thoracic spinal cord hemisection.
Rosario Gulino, Salvatore Andrea Lombardo, Antonino Casabona, Giampiero Leanza, Vincenzo Perciavalle
Brain Research null
A role for the p75 neurotrophin receptor in axonal degeneration and apoptosis induced by oxidative stress.
Kraemer, BR; Snow, JP; Vollbrecht, P; Pathak, A; Valentine, WM; Deutch, AY; Carter, BD
The Journal of Biological Chemistry null
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