AB10554
Anti-Tbr1 Antibody
from rabbit, purified by affinity chromatography
Synonyme(s) :
T-box, brain, 1, T-brain-1, T-box brain protein 1
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
mouse
Réactivité de l'espèce (prédite par homologie)
canine (based on 100% sequence homology), canine, bovine, opossum, horse, rat, pig
Technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... TBR1(10716)
Description générale
T-box brain protein 1 (UniProt: Q64336; also known as T-brain-1, TBR-1, TES-56) is encoded by the Tbr1 gene (Gene ID: 21375) in murine species. Tbr1 is a homodimeric nuclear protein that serves as a transcriptional repressor and is involved in multiple aspects of cortical development, including neuronal migration, laminar and areal identity, and axonal projection. As transcriptional repressor of FEZ Family Zinc Finger 2 (FEZF2), it blocks the formation of the corticospinal (CS) tract from layer 6 projection neurons, thereby restricting the origin of CS axons specifically to layer 5 neurons. Its T-box DNA binding domain is localized in amino acids 213-393. Its expression is observed in developing and adult cerebral cortex and in olfactory bulbs. It is detected around day 10 of embryonic development in the preplate and at day 12.5 in the cortical plate. It is highly expressed in glutamatergic early-born cortical neurons. Tbr1 null mice exhibit severe defects of frontal cortex and layer 6 differentiation. (Ref.: Han, W., et al. (2011). Proc. Natl. Acad. Sci. USA. 108(7); 3041-3046; Bedogni, F., et al (2010). Proc. Natl Acad. Sci. USA. 107(29); 13129-13134; Hevner, RF., et al. (2001). Neuron. 29(2); 353-366).
Spécificité
This rabbit polyclonal antibody detects T-box brain protein 1 (Tbr1).
Immunogène
KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of mouse T-box brain protein 1 (Tbr1).
KLH-conjugated linear peptide corresponding to mouse Tbr1 near the N-terminus.
Application
Quality Control Testing
Evaluated by Immunohistochemistry (Paraffin) in Mouse brain tissue sections.Immunohistochemistry (Paraffin) Analysis: A 1:400 dilution of this antibody detected Tbr1 in Mouse cerebral cortex and cerebellum tissue sections.
Tested Applications
Western Blotting Analysis: A 1:500 dilution from a representative lot detected Tbr1 in Mouse fetal brain tissue lysate.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Immunohistochemistry (Paraffin) in Mouse brain tissue sections.Immunohistochemistry (Paraffin) Analysis: A 1:400 dilution of this antibody detected Tbr1 in Mouse cerebral cortex and cerebellum tissue sections.
Tested Applications
Western Blotting Analysis: A 1:500 dilution from a representative lot detected Tbr1 in Mouse fetal brain tissue lysate.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Qualité
Evaluated by Immunohistochemistry in mouse frontal cortex tissue.
Immunohistochemistry Analysis: 1:400 dilution of this antibody detected Tbr1 in mouse frontal cortex tissue.
Immunohistochemistry Analysis: 1:400 dilution of this antibody detected Tbr1 in mouse frontal cortex tissue.
Description de la cible
~76 kDa observed; 73.94 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Forme physique
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Recommended storage: +2°C to +8°C.
Remarque sur l'analyse
Control
Mouse frontal cortex tissue
Mouse frontal cortex tissue
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Epigenomic Analysis of Multilineage Differentiation of Human Embryonic Stem Cells.
Xie, Wei, et al.
Cell (2013)
J Ni et al.
International journal of molecular medicine, 35(6), 1755-1760 (2015-04-08)
Knee osteoarthritis (OA) is the most prevalent type of OA and the cytokine, oncostatin M (OSM), may contribute to the pathogenesis of OA. However, the exact role of OSM in the development of knee OA and the underlying mechanisms are
Xue Li et al.
Cerebral cortex (New York, N.Y. : 1991), 30(7), 3960-3976 (2020-02-03)
De novo microdeletion of chromosome 2p15-16.1 presents clinically recognizable phenotypes that include mental retardation, autism, and microcephaly. Chromosomal maintenance 1 (CRM1) is a gene commonly missing in patients with 2p15-16.1 microdeletion and one of two genes found in the smallest
Christine Sauerland et al.
Cerebral cortex (New York, N.Y. : 1991), 28(1), 145-157 (2017-12-19)
A hallmark of mammalian brain evolution is the emergence of the neocortex, which has expanded in all mammalian infraclasses (Eutheria, Marsupialia, Monotremata). In eutherians, neocortical neurons derive from distinct neural stem and progenitor cells (NPCs). However, precise data on the
Ilaria Favicchia et al.
Frontiers in molecular neuroscience, 14, 663598-663598 (2021-09-24)
Tbx1 mutant mice are a widely used model of 22q11.2 deletion syndrome (22q11.2DS) because they manifest a broad spectrum of physical and behavioral abnormalities that is similar to that found in 22q11.2DS patients. In Tbx1 mutants, brain abnormalities include changes
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