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69670-M

Millipore

His•Bind® Resin

Synonyme(s) :

uncharged IDA agarose beads

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

Forme

liquid

Fabricant/nom de marque

Novagen®

Conditions de stockage

do not freeze

Application(s)

life science and biopharma

Température de stockage

2-8°C

Description générale

His•Bind Resin is an uncharged IDA agarose resin. The binding capacity is approximately 8 mg/ml bed volume. His•Bind Resin is well suited to small to medium scale purifications using either gravity flow columns or batch methods.

Application

His•Bind® Resin have been used: in purification of His•Tag® fusion proteins by metal chelation chromatography, for purification of the bacterially produced OCRL1WT/VAR phosphatase domain to study heterogeneity in Lowe Syndromein protein purification to study anaerobic phloroglucinol degradation by Clostridium scatologenes for isolation of His6-tagged proteins for studying the role of human repressor activator protein 1 (RAP1) and glial fibrillary acidic protein (GFAPɛ)proteins in increasing γ-secretase activity in a yeast model system.

Actions biochimiques/physiologiques

His•Bind® resin helps in the purification of proteins containing the His-tagged sequence by the process of metal chelation chromatography. It utilizes the charge buffer provided in the His•Bind® Buffer Kit, that binds the His-tagged sequence effectively with Ni2+ or Co2+ cations that are immobilized onto the His•Bind® resin. The target protein can be easily recovered by eluting with imidazole. The His•Bind® Resin is designed to be regenerated and reused multiple times, making it a cost-effective solution. Its versatility allows for protein purification under gentle, non-denaturing conditions, or in the presence of either guanidine or urea, depending on the specific requirements of the purification process.

Caractéristiques et avantages

  • Reusable many times
  • Compatible with His•Bind® Buffer Kit
  • Compatible with 1 mM THP

Avertissement

Toxicity: Standard Handling (A)

Informations légales

HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Classification des risques

Flam. Liq. 3

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

86.0 °F

Point d'éclair (°C)

30 °C

Certificats d'analyse (COA)

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