688225

Caspase-1 Substrate VI, Fluorogenic

Fluorogenic caspase-1 substrate.

• Synonyme(s) : Caspase-1 Substrate VI, Fluorogenic, Z-YVAD-AFC
• Formule empirique (notation de Hill): C₃₃H₃₆F₃N₅O₁₀
• Numéro CAS: 219137-85-6
• Poids moléculaire : 719.66
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.77

Propriétés

• Niveau de qualité: 100
• Pureté: 98% (HPLC)
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated; protect from light
• Couleur: white to off-white
• Solubilité: DMSO: 5 mg/mL
• Fluorescence: λ_ex ~400 nm; λ_em ~505 nm
• Conditions d'expédition: ambient
• Température de stockage: −20°C
• InChI: 1S/C33H36F3N5O10/c1-15(2)28(41-31(49)23(38-17(4)42)11-18-5-8-20(43)9-6-18)32(50)37-16(3)29(47)40-24(14-26(44)45)30(48)39-19-7-10-21-22(33(34,35)36)13-27(46)51-25(21)12-19/h5-10,12-13,15-16,23-24,28,43H,11,14H2,1-4H3,(H,37,50)(H,38,42)(H,39,48)(H,40,47)(H,41,49)(H,44,45)/t16-,23-,24-,28-/m0/s1
• Clé InChI: GWKJISJKNKIJNP-DEQDFGQZSA-N

Description

Description générale

Fluorogenic ICE substrate. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. AFC (7-amino-4-trifluoromethylcoumarin) based fluorometric substrates are superior to AMC (7-amino-4-methylcoumarin) substrates because released AFC has a greater Stoke′s shift than AMC. Excitation/emission maxima (nm) for AFC and AMC are 400/505 and 380/460, respectively.

Fluorogenic caspase-1 substrate. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.

Actions biochimiques/physiologiques

Cell permeable: no

Primary Target
Fluorogenic caspase-1 substrate

Product does not compete with ATP.

Reversible: no

Avertissement

Toxicity: Standard Handling (A)

Séquence

Ac-Tyr-Val-Ala-Asp-AFC

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Autres remarques

Jones, R.A., et al. 1998. Hepatology 27, 1632.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Walker, N.P., et al. 1994. Cell 78, 343.
Thornberry, N.A., et al. 1992. Nature 356, 768.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable