605160

Thrombin, Plasminogen-Free, Bovine

• Synonyme(s) : Thrombin, Plasminogen-Free, Bovine
• Numéro CAS: 9002-04-4
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.25

Propriétés

• Forme: lyophilized
• Niveau de qualité: 100
• Activité spécifique: ≥1000 NIH units/mg protein
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated
• Solubilité: water: 1 mg/mL
• Activité étrangère: Plasminogen, none detected (by rocket IEP)
• Conditions d'expédition: ambient
• Température de stockage: −20°C

Description

Description générale

Native, bovine thrombin. Key enzyme that activates factor XIII and converts fibronogen into fibrin. Note: 1 KU = 1000 units.

Native, bovine thrombin. Key enzyme that activates factor XIII and converts fibronogen into fibrin. Serine protease formed in the plasma during the coagulation process by activation of prothrombin by a complex of Factor Xa, Factor Va, phospholipid, and Ca²⁺. Thrombin cleaves Arg-Gly bonds in specific sequences only. The best characterized activity of thrombin is the conversion of fibrinogen to fibrin in the clotting process. Inhibited by AEBSF (Cat. No. 101500) Antithrombin (Cat. No. 169756), PMSF (Cat. No. 52332), and TLCK (Cat. No. 616382). Note: 1 KU = 1000 units.

Avertissement

Toxicity: Harmful (C)

Définition de l'unité

One unit is determined by comparison with a standard curve prepared using the Bureau of Biologics standard thrombin.

Forme physique

Lyophilized from 200 mM NaCl, 50 mM sodium citrate, 0.1% PEG-8000, pH 6.5.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.

Remarque sur l'analyse

Complete activation from homogeneous bovine prothrombin by SDS-PAGE

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H315,H319,H334,H335
• Conseils de prudence: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable