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Anti-KDEL Mouse mAb (10C3)

liquid, clone 10C3, Calbiochem®

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

10C3, monoclonal

Forme

liquid

Contient

≤0.1% sodium azide as preservative

Espèces réactives

mouse, sheep, canine, hamster, Drosophila, rat, guinea pig, human, monkey, yeast, rabbit, porcine

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
avoid repeated freeze/thaw cycles

Isotype

IgG2a

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

mouse ... Kdelr1(68137)

Description générale

Protein G purified mouse monoclonal antibody. Recognizes the KDEL Grp78 (BiP) and Grp94 proteins.
Recognizes the Grp78 (BiP) and Grp94 proteins.
This Anti-KDEL Mouse mAb (10C3) is validated for use in Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of KDEL.

Immunogène

Rat
a synthetic peptide (SEKDEL) corresponding to amino acids 649-654 of rat Grp78, conjugated to KLH

Application

Immunoblotting (4 µg/ml, colorimetric)

Immunocytochemistry (5 µg/ml)

Immunoprecipitation (5 µg/ml)

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Highly Toxic (H)

Forme physique

In PBS, 50% glycerol.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Remarque sur l'analyse

Positive Control
RK-13 cells, Mouse liver microsomes, Rat liver microsomes, Heat shocked HeLa cells

Autres remarques

Also recognizes unidentified proteins at ~55 kDa and ~43 kDa by immunoblotting. Does not cross-react with Hsp70. Variables associated with assay conditions will dictate the proper working dilution.
Kuznetsov, G., et al. 1994. J. Biol. Chem.269, 22990.
Huovila, A.-P., et al. 1992. J. Cell Biol. 118, 1305.
Munro, S., and Pelham, H.R.B. 1987. Cell48, 899.
Munro, S., and Pelham, H.R.B. 1986. Cell46, 291.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Michael A Robichaux et al.
Disease models & mechanisms, 15(5) (2022-03-12)
The P23H mutation in rhodopsin (Rho), the rod visual pigment, is the most common allele associated with autosomal-dominant retinitis pigmentosa (adRP). The fate of misfolded mutant Rho in rod photoreceptors has yet to be elucidated. We generated a new mouse
Gary P Brennan et al.
PloS one, 8(1), e54491-e54491 (2013-01-30)
14-3-3 proteins are ubiquitous molecular chaperones that are abundantly expressed in the brain where they regulate cell functions including metabolism, the cell cycle and apoptosis. Brain levels of several 14-3-3 isoforms are altered in diseases of the nervous system, including
Tobias Becker et al.
iScience, 24(12), 103521-103521 (2021-12-18)
Protein AMPylation is a posttranslational modification with an emerging role in neurodevelopment. In metazoans two highly conserved protein AMP-transferases together with a diverse group of AMPylated proteins have been identified using chemical proteomics and biochemical techniques. However, the function of

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