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Key Documents

407303

Sigma-Aldrich

γ-Interferon, Mouse, Recombinant, E. coli

Synonyme(s) :

IFNG, Type II IFN

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

Pureté

≥95% (SDS-PAGE)

Niveau de qualité

Forme

liquid

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
avoid repeated freeze/thaw cycles

Solubilité

water: soluble

Numéro d'accès Protein ID

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Température de stockage

−70°C

Informations sur le gène

mouse ... Ifng(15978)

Description générale

Note: 1 KU = 1000 units.
Research area: Immunology and Cytokines

Recombinant, mouse γ-interferon, consisting of 134 amino acids, expressed in E. coli as a single, non-glycosylated polypeptide.

Application

γ-Interferon has been used to assess the response of wild-type and cytosolic phospholipase A2 (cPLA2)-deficient cells to IFN-mediated signals. It has also been used to study the signaling pathway associated with IFN-γ and to explore the potential role of β-Arrestin1 in the dephosphorylation of signal transducer and activator of transcription 1 (STAT1).

Actions biochimiques/physiologiques

Interferon-gamma (IFN-γ), a proinflammatory cytokine, exhibits a significant impact on innate and adaptive immunity in terms of combatting intracellular infections and facilitating tumor control. It has a significant impact on various immunological pathways. IFN-γ influences the following biological processes:

Antigen Processing and Presentation: IFN-γ enhances the expression of molecules involved in antigen presentation. This helps in the efficient activation of T cells, leading to the initiation of adaptive immunity.

Reactive Oxygen Species (ROS): IFN-γ stimulates the production of ROS by promoting the assembly of NADPH oxidase subunits. These ROS possess strong microbicidal effects, aiding in the defense against invading pathogens.

Autophagy: IFN-γ induces the expression of proteins involved in autophagy, facilitating the clearance of intracellular pathogens and contributing to immune defense.

Apoptosis: IFN-γ triggers apoptotic cell death by increasing the expression of molecules associated with the apoptotic pathway.

Cell Migration and Leukocyte Traffic: IFN-γ promotes the expression of chemokines and adhesion molecules, facilitating immune cell migration and contributing to the initiation of inflammatory responses.

Antiviral Mediators: By inducing the expression of various antiviral proteins, IFN-γ counteracts viral infections at multiple stages of the viral life cycle.

Avertissement

Toxicity: Standard Handling (A)

Forme physique

In 10 mM PBS, pH 7.4.

Notes préparatoires

Dilute only in buffers containing protein such as 0.1% BSA.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Autres remarques

Zantl, N., et al. 1998. Infect. Immun. 66, 2300.
Familletti, P.C., et al. 1981. Methods Enzymol. 78, 387.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Christin Pelzel et al.
Molecular cell, 50(1), 149-156 (2013-04-16)
Signal transducer and activator of transcription 1 (STAT1) is activated by tyrosine phosphorylation upon interferon-γ (IFNγ) stimulation, which results in the expression of genes with antiproliferative and immunomodulatory functions. The inactivation of STAT1 occurs through tyrosine dephosphorylation by the tyrosine

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