368059-M

Granzyme B Substrate II, Fluorogenic

A fluorogenic substrate for the detection of granzyme B and caspase-8 activity.

• Synonyme(s) : Granzyme B Substrate II, Fluorogenic, Z-IETD-AFC, Caspase-8 Substrate II
• Formule empirique (notation de Hill): C₃₇H₄₂F₃N₅O₁₃
• Poids moléculaire : 821.75
• Code UNSPSC : 12352200

Propriétés

• Niveau de qualité: 100
• Pureté: ≥97% (HPLC)
• Forme: lyophilized solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; protect from light
• Couleur: white
• Solubilité: DMSO: 1 mg/mL
• Fluorescence: λ_ex ~400 nm; λ_em ~505 nm
• Température de stockage: −20°C

Description

Description générale

A fluorogenic substrate for the detection of granzyme B and caspase-8 activity. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.

A fluorogenic substrate for the detection of granzyme B. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore (excitation maximum: ~400 nm; emission maximum: ~505 nm).

Actions biochimiques/physiologiques

Primary Target
Fluorogenic substrate for the detection of granzyme B and caspase-8 activity

Avertissement

Toxicity: Standard Handling (A)

Séquence

Z-Ile-Glu-Thr-Asp-AFC

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Autres remarques

Karahashi, H., and Amano, F. 2000. Biol. Pharm. Bull. 23, 140.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable