324682

Elastase, Porcine Pancreas, High Purity, Crystallized

Elastase, Porcine Pancreas, High Purity, Crystallized, CAS 39445-21-1, is a serine protease that hydrolyzes proteins and peptides, especially at bonds adjacent to neutral amino acid residues.

• Synonyme(s) : Elastase, Porcine Pancreas, High Purity, Crystallized
• Numéro CAS: 39445-21-1
• Numéro de classification (Commission des enzymes): 3.4.21.36 (BRENDA, IUBMB)
• Numéro MDL: MFCD00130998
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Niveau de qualité: 100
• Forme: lyophilized
• Activité spécifique: ≥25 units/mg protein
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated
• Solubilité: distilled water: 10 mg/mL
• Activité étrangère: Chymotrypsin ≤0.2%
• Conditions d'expédition: ambient
• Température de stockage: −20°C

Description

Description générale

Native elastase from porcine pancreas. A serine protase that catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin and of a number of synthetic substrates containing aspartic acid, phenylalanine, or tyrosine. Inhibited by DFP, elastinal, and α₂-macroglobulin.

Native elastase from porcine pancreas. Catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin, and of a number of synthetic substrates containing aspartic acid, glutamic acid, phenylalanine, or tyrosine. Preferentially cleaves peptide bonds at the carbonyl end of amino acid residues with small hydrophobic side chains, such as glycine, valine, leucine, isoleucine, and particularly alanine. Inhibited by DFP, elastinal, and α2-macroglobulin. Has an optimal pH of 7.8-8.5; pI = 9.5. Note: Elastase tends to adhere to glass. Hence, use of siliconized glassware is recommended.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that will hydrolyze 1.0 mol of Suc-Ala-Ala-Pro-Abu-pNA (Cat no.324699) per min at 25°C, pH 8.0.

Forme physique

Lyophilized from 50 mM Trehalose, 1 mM Acetic Acid.

Reconstitution

Following reconstitution, elastase may be stored at 4°C at pH 6.0 for long term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4°C, pH 6.0.

Autres remarques

Largman, C. et al. 1976. Biochemestry15, 2491.
Mandl, I. 1962. Methods in Enzymol.5, 665.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H315,H319,H334,H335
• Conseils de prudence: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3