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Sigma-Aldrich

Anti-Dopamine D₅ Receptor Rabbit pAb

lyophilized, Calbiochem®

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

serum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

lyophilized

Ne contient pas

preservative

Espèces réactives

human, rat

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze

Isotype

IgG

Conditions d'expédition

ambient

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... DRD5(1816)

Description générale

Anti-Dopamine D5 Receptor, rabbit polyclonal, recognizes the dopamine D5 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB, FC, and IHC.
Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~49 kDa D5 dopamine receptor protein.
Recognizes the dopamine D5 receptor in rat brain. Does not cross-react with other dopamine receptors.

Immunogène

Human
a synthetic peptide (LPPGSNGTAYC) corresponding to amino acids 2-10 of human dopamine D₅ receptor, conjugated to carrier protein

Application

ELISA (see comments)

Flow Cytometry (1:100, see application references)

Immunoblotting (1:200)

Immunohistochemistry (1:2500)

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Undiluted serum.

Reconstitution

Reconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).

Remarque sur l'analyse

Positive Control
Rat brain

Autres remarques

For immunohistochemistry, a 1:2500 dilution of the reconstituted antiserum demonstrated staining of specific cells in various regions of rat brain sections, including Cortex layer 5 and parafascicular nucleus. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.
McKenna, F., et al. 2002. J. Neuroimmunol.132, 34.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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The International journal of developmental biology, 66(1-2-3), 263-267 (2021-12-10)
Even before the first synapses appear, neurotransmitters and their receptors are present in the developing brain, regulating the cell fate of neuronal progenitors in neurogenic niches, such as the lateral ventricle. In particular, dopamine appears to play a pivotal role
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