23-028-M

IkBa Protein, activated, 10 µg

Activated, full length, recombinant human IκBα. N-terminal c-Myc, 6His-tagged. Phosphorylated in vitro using IKKα, active. For use in Enzyme Assays. Behaves as a substrate in ubiquitin assays.

• Synonyme(s) : NF-kappa-B inhibitor alpha, I-kappa-B-alpha, Major histocompatibility complex enhancer-binding protein MAD3
• Code UNSPSC : 12352202
• eCl@ss : 32160405
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: human
• Niveau de qualité: 100; 300
• Produit recombinant: expressed in E. coli
• Fabricant/nom de marque: Upstate^®
• Technique(s): activity assay: suitable
• Numéro d'accès UniProt: M69043

Description

Description générale

IκBα is a cellular protein that inhibits the transcription factor NF-κB. NF-κB plays a central role in cellular stress and inflammatory responses by controlling cytokine-inducible gene expression and lymphocyte stimulation by antigens. In non-stimulated cells NF-κB is sequestered in the cytoplasm via the inhibitory protein IκBα, which obscures the nuclear localization signal of NF-κB and also blocks its ability to bind DNA. In response to various stimuli, including viral infection, ultraviolet radiation and inflammatory cytokines, IκBα is phosphorylated by the protein kinase complex IKK, which triggers its rapid degradation by ubiquitin mediated proteolysis. The liberated NF-κB then translocates to the nucleus and activates the expression of target genes. Mutations in the gene for IκBα result in the functional impairment of NF-κB which causes a decreased production of proinflammatory cytokines and certain interferons, rendering patients susceptible to infection.

N-terminal GST-tagged, recombinant human Smurf1 full length, expressed in E coli.

Application

Ubiquitination Cascade Comoponent: Substrate

Forme physique

Purified using immobilized metal affinity chromatography.

Autres remarques

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable