219404

Cathepsin H, Human Liver

Cathepsin H, Human Liver, CAS 60748-73-4, is native cathepsin H from human liver that functions both as an aminopeptidase and as an endopeptidase.

• Synonyme(s) : Cathepsin H, Human Liver, CTSH, cat H, procathepsin h
• Numéro CAS: 60748-73-4
• Numéro de classification (Commission des enzymes): 3.4.22.16 (BRENDA, IUBMB)
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Niveau de qualité: 100
• Forme: liquid
• Activité spécifique: ≥200 mU/mg protein
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Technique(s): activity assay: suitable
• Couleur: colorless
• pH: 4.5
• Solubilité: soluble; water: soluble
• Adéquation: suitable for Western blot
• Numéro d'accès NCBI: NM_004390
• Numéro d'accès UniProt: P09668
• Conditions d'expédition: wet ice
• Température de stockage: −70°C
• Informations sur le gène: human ... CTSH(1512)

Description

Description générale

Research area: Cell signaling

Native Cathepsin H, from the human liver, is the only cathepsin with mono-aminopeptidase activity.

Application

Cathepsin H has been used to determine the potential of aziridinyl peptides to act as inhibitors of this protein and potentially explore their therapeutic applications.

Actions biochimiques/physiologiques

Cathepsin H, derived from human liver, serves dual functions as both an aminopeptidase and an endopeptidase. It plays a significant role in the catabolism of proteins within lysosomes. Cathepsin H is classified as an endoaminopeptidase because it can hydrolyze proteins, N-terminal substituted proteins, amino acid derivatives, and peptides with different chain lengths and N-terminal free amino acid derivatives. This protein is also observed to exhibit amidase and esterase activity.

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol H-Arg-βNA per min at 40°C, pH 6.8.

Forme physique

In 100 mM NaCl, 20 mM sodium acetate buffer, 1 mM EDTA, pH 4.5.

Notes préparatoires

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Autres remarques

Note: 1 mU = 1 milliunit.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2