217721

RO-3306

≥95% (HPLC), liquid, Cdk1 inhibitor, Calbiochem^®

• Synonyme(s) : InSolution Cdk1 Inhibitor IV, RO-3306, 5-(6-Quinolinylmethylene)-2-((2-thienylmethyl)amino)-4(5H)-thiazolone, 5-(Quinolin-6-ylmethylene)-2-(thiophen-2-ylmethylamino)thiazol-4(5H)-one, 5-(Quinolin-6-ylmethylene)-2-(thiophen-2-ylmethylamino)thiazol-4(5H)-one, 5-(6-Quinolinylmethylene)-2-((2-thienylmethyl)amino)-4(5H)-thiazolone
• Formule empirique (notation de Hill) : C₁₈H₁₃N₃OS₂
• Poids moléculaire : 351.45
• Numéro MDL: MFCD17392573
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.77

Propriétés

• Nom du produit: Cdk1 Inhibitor IV, RO-3306, InSolution, ≥95%
• Niveau de qualité: 100
• Essai: ≥95% (HPLC)
• Forme: liquid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles; desiccated (hygroscopic); protect from light
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Chaîne SMILES: [s]1c(ccc1)CNC2=NC(=O)\C(=C\c3cc4c(nccc4)cc3)\S2
• InChI: 1S/C18H13N3OS2/c22-17-16(24-18(21-17)20-11-14-4-2-8-23-14)10-12-5-6-15-13(9-12)3-1-7-19-15/h1-10H,11H2,(H,20,21,22)/b16-10-
• Clé InChI: XOLMRFUGOINFDQ-YBEGLDIGSA-N

Description

Description générale

A cell-permeable quinolinyl thiazolinone compound that acts as a potent and ATP-competitive inhibitor of Cdk1 (K_i = 35 nM and 110 nM for Cdk1/B1 and Cdk1/A, respectively). It affects Cdk2/E, PKCδ, and SGK only at much higher concentrations (K_i = 340, 318, and 497 nM, respectively), and shows little effect against Cdk4/D and six other commonly studied kinases (K_i =2 M). Short-term treatment for up to 20 hrs results in fully reversible G2/M cell cycle arrest, while prolonged treament (>48 hrs) results in apoptotic cell death in proliferating cancer cells, but not in nontumorigenic epithelial cell lines. The solid form of this compound (Cat. No. 217699) is also available.

Actions biochimiques/physiologiques

Cell permeable: yes

Primary Target
Cdk1/B1 and Cdk1/A

Reversible: yes

Secondary Target
Cdk2/E, PKCδ, and SGK

Target K_i: 35 nM and 110 nM for Cdk1/B1 and Cdk1/A,

Conditionnement

Packaged under inert gas

Avertissement

Toxicity: Irritant (B)

Forme physique

A 10 mM (2 mg/569 L) solution of Cdk1 Inhibitor IV, RO-3306 (Cat. No. 217699) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 6 months at -70°C.

Autres remarques

Vassilev, L.T., et al. 2006. Proc. Natl. Acad. Sci. USA103, 10660.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): 188.6 °F - closed cup - (refers to pure substance)
• Point d'éclair (°C): 87 °C - closed cup - (refers to pure substance)