208921

Calmodulin Kinase IINtide, Myristoylated

≥95% (HPLC), solid, CaMK II inhibitor, Calbiochem^®

• Synonyme(s) : Calmodulin Kinase IINtide, Myristoylated, Myr-N-GGGKRPPKLGQIGRAKRVVIEDDRIDDVLK-OH
• Formule empirique (notation de Hill): C₂₅₆H₂₇₅N₄₇O₄₃
• Poids moléculaire : 4698.21
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.54

Propriétés

• product name: Calmodulin Kinase IINtide, Myristoylated, The myristoylated form of CaMK IINtide.
• Niveau de qualité: 100
• Pureté: ≥95% (HPLC)
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated (hygroscopic)
• Couleur: white
• Solubilité: DMSO: 5 mg/mL
• Conditions d'expédition: ambient
• Température de stockage: −20°C

Description

Description générale

The myristoylated form of CaMK IINtide (Cat. No. 208920). The peptide has been modified at the amino terminal lysine with the addition of three glycine residues and myristoylated to improve cell-permeability.

The myristoylated form of CaMK IINtide (Cat. No. 208920), a potent, specific inhibitor of Ca²⁺/Calmodulin kinase II (CaMK II) (IC₅₀ = 50 nM for total and Ca²⁺-independent CaMK II activity). The peptide sequence corresponds to the inhibitory domain of the CaMK II inhibitory protein, CaMK IIN. Exhibits inhibitory activity across converged species, including rat brain, goldfish brain, and Drosophila (IC₅₀ = 100-400 nM). Shown to completely inhibit the phosphorylation of GluR1 fusion protein at a concentration of 1 µM. Does not inhibit CaMK I, CaMK IV, CaMKK, PKA, or PKC. The peptide has been modified at the amino terminal lysine with the addition of three glycine residues and myristoylated to improve cell-permeability.

Actions biochimiques/physiologiques

Cell permeable: yes

Primary Target
Calmodulin-Dependent Protein Kinase (CaM Kinase)-2

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 50 nM against cam Kinase-2

Conditionnement

Packaged under inert gas

Avertissement

Toxicity: Standard Handling (A)

Séquence

Myr-N-Gly-Gly-Gly-Lys-Arg-Pro-Pro-Lys-Leu-Gly-Gln-Ile-Gly-Arg-Ala-Lys-Arg-Val-Val-Ile-Glu-Asp-Asp-Arg-Ile-Asp-Asp-Val-Leu-Lys-OH

Forme physique

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at-20°C.

Autres remarques

Sodering, T.R., et al. 2001. J. Biol. Chem.276, 3719.
Chang, B.H., et al. 1998. Proc. Natl. Acad. Sci. USA95, 10890.
Pereda, A.E., et al. 1998. Proc. Natl. Acad. Sci. USA95, 13272.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable