171263-M

AMPK Signaling Activator IX, F17

The AMPK Signaling Agonist, F17, also referenced under CAS 280112-24-5, controls the biological activity of AMPK. This small molecule/inhibitor is primarily used for Biochemicals applications.

• Synonyme(s) : AMPK Signaling Activator IX, F17, 4-hydroxy-1-isobutyl-N-(5-methylthiazol-2-yl)-2-oxo-1,2-dihydroquinoline-3-carboxamide
• Formule empirique (notation de Hill): C₁₈H₁₉N₃O₃S
• Numéro CAS: 280112-24-5
• Poids moléculaire : 357.43
• Code UNSPSC : 12352200

Propriétés

• Essai: ≥95% (HPLC)
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; protect from light
• Couleur: off-white
• Solubilité: DMSO: 5 mg/mL, clear, colorless
• Température de stockage: 2-8°C
• InChI: 1S/C18H19N3O3S/c1-10(2)9-21-13-7-5-4-6-12(13)15(22)14(17(21)24)16(23)20-18-19-8-11(3)25-18/h4-8,10,22H,9H2,1-3H3,(H,19,20,23)
• Clé InChI: QSZAFLFWFQTCJK-UHFFFAOYSA-N

Description

Description générale

A cell-permeable 4-hydroxy-2-oxo quinoline carboxamide that acts as an agonist of AMPK signaling. It is shown to reduce fat storage in C. elegans (EC₅₀ ~ 0.1 µM) and reduce the expression level of fat-7 (a C. elegans ortholog of mammalian stearoyl-CoA desaturase-1), through aak-1 (an ortholog for the AMPK-α1 catalytic subunit) and K08F8.2 (a transcription factor) dependent mechanisms. In addition, it markedly reduces the number of lipid droplets in HepG2 human hepatocarcinoma cells at 25 µM, and elicits the phosphorylation and inactivation of acetyl coenzyme A carboxylase (ACC, the enzyme that catalyzes the rate-limiting step in de novo fatty acid synthesis). F17-induced ACC phosphorylation can be abrogated by simultaneous treatment with an AMPK inhibitor compound C (Cat. No. 171260).

Avertissement

Toxicity: Standard Handling (A)

Notes préparatoires

Warming to 55-60°C is required for complete solubilization.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Autres remarques

Lemieux, G.A., et al. 2011. Nat. Chem. Biol.7, 206.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable