15-115
AMPK Pathway Explorer Antibody MiniPack
1 mg/mL, Upstate®
Synonyme(s) :
AMP-Activated Protein Kinase
About This Item
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
rat (07-350SP, 07-363SP, 07-681SP), mouse (07-350SP), human (07-350SP, 07-363SP, 07-681SP)
Fabricant/nom de marque
Upstate®
Concentration
1 mg/mL
Technique(s)
activity assay: suitable (kinase and phosphatase)
western blot: suitable
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Informations sur le gène
human ... PRKAA1(5562)
mouse ... Hsf3(245525)
Description générale
Each Pathway Explorer Antibody Minipack contains three related antibodies as part of a signaling cascade or a combination of total and phosphorylated forms of key signaling targets. Each of the three antibodies are 30% the original pack size. Full size versions of each of the Pathway Explorer antibodies are available for sale individually under the same catalog number with the removal of “SP” off of each one (e.g. 05-591SP can be ordered as 05-591).
AMPK:
AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kDa) and non-catalytic beta and gamma subunits. Alpha subunit has at least two isoforms (alpha 1 and alpha 2) which differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity.
*See full size versions for corresponding references.
Spécificité
07-363SP: AMP-Activated Protein Kinase alpha 2 (AMPKα2).
07-350SP: AMPKα1
Varies. See individual Data Sheets
Immunogène
Application
Signaling
Apoptosis & Cancer
Insulin/Energy Signaling
Conditionnement
Composants
07-363SP Anti-AMPKα2; 60 µg
07-681SP Anti-phospho-AMPKα (Thr172); 60 µg
Qualité
Western Blot Analysis:
0.5-2 μg/mL of this lot detected phospho-AMPK α in RIPA lysates from overnight serum starved HEK293 cells and partially purified rat liver AMPK. Signal was reduced after treatment of lysate with λ-phosphatase.
07-363SP: Routinely evaluated by immunoblot in a rat liver preparation.
Western Blot Analysis:
1-2 μg/mL of this lot detected AMPK α2 in a rat liver preparation.
07-350SP: Routinely evaluated by immunoblot in RIPA lysates from non-stimulated A431 cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected AMPK α1 in RIPA lysates from non-stimulated A431 cells.
Description de la cible
Forme physique
Stockage et stabilité
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Remarque sur l'analyse
For 07-681SP use HEK293 cells or rat liver AMPK.
For 07-363SP use Rat liver preparation.
For 07-350SP use A431cell lysate, or lysate of HeLa cells treated with heat shock.
Informations légales
Clause de non-responsabilité
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Certificats d'analyse (COA)
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