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Key Documents

12-301

Sigma-Aldrich

Non-Stimulated A431 Cell Lysate

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About This Item

Code UNSPSC :
41105330
eCl@ss :
32011807
Nomenclature NACRES :
NA.41

Source biologique

human

Niveau de qualité

Forme

liquid

Fabricant/nom de marque

Upstate®

Technique(s)

western blot: suitable

Conditions d'expédition

dry ice

Description de la lignée cellulaire

A431

Qualité

Routinely evaluated as a positive control for several of Upstates antibodies.

Forme physique

modified RIPA buffer (50mM Tris-HCl, pH 7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM NaCl, 1mM EDTA, 1mM PMSF, 1μg/ml each of aprotinin, leupeptin, pepstatin, 1mM Na3VO4, 1mM NaF) and diluted with non-reducing sample buffer (31mM Tris-HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% bromphenol blue).

Stockage et stabilité

1 year at -20°C

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogrammes

Exclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Aquatic Chronic 3 - Eye Irrit. 2

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

D J Flint et al.
Journal of molecular endocrinology, 36(3), 435-448 (2006-05-25)
Insulin-like growth factor-binding protein 5 (IGFBP-5) mediates involution of the mammary gland. The decrease in DNA content and mammary gland weight which accompanies involution was inhibited by prolactin (PRL) in wild-type but not transgenic mice expressing IGFBP-5. Phospho-STAT5 protein levels
Yu Shi et al.
Molecular and cellular biology, 23(21), 7732-7741 (2003-10-16)
MK5 (mitogen-activated protein kinase [MAPK]-activated protein kinase 5), also designated PRAK (p38-regulated and -activated kinase), was deleted from mice by homologous recombination. Although no MK5 full-length protein and kinase activity was detected in the MK5 knockout mice, the animals were
Johannes Witt et al.
BMC systems biology, 3, 71-71 (2009-07-18)
Biological effects of nuclear factor-kappaB (NF kappaB) can differ tremendously depending on the cellular context. For example, NF kappaB induced by interleukin-1 (IL-1) is converted from an inhibitor of death receptor induced apoptosis into a promoter of ultraviolet-B radiation (UVB)-induced
Sandra Barisic et al.
Biochemical pharmacology, 80(4), 439-447 (2010-05-11)
Activation status of Tyr-kinase Src as well as of the transcription factor NFkappaB is a decisive criterion for the onset of cancer and in conveying radio-resistance. While the activation status of Src is Tyr phosphorylation-dependent, NFkappaB activation requires Ser phosphorylation

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