1.09217

Gram′s safranine solution

for the Gram staining method

• Code UNSPSC : 41116130
• Nomenclature NACRES : NA.74

Propriétés

• Niveau de qualité: 400
• Forme: liquid
• IVD: for in vitro diagnostic use
• Technique(s): microbe id | staining: suitable
• Température de transition: flash point 49 °C
• Densité: 0.98 g/cm³ at 20 °C
• Application(s): clinical testing; diagnostic assay manufacturing; hematology; histology
• Température de stockage: 15-25°C

Description

Description générale

This ready-to-use Gram′s safranine solution-for the Gram staining method, is used in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram′s crystal violet solution (Product number 1.09218) - an aniline dye - in the first step. After the treatment with iodinesolution (Lugol′s solution stabilized with PVP, Product number 1.00567), a dye-iodine complex will form. During the decolorizing step (Gram′s decolorizing solution, 1.10218), this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue-violet. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram′s safranin solution and appear pink to red.
A bottle with 500 ml Gram′s safranin solution is sufficient for staining up to 250 slides, it is also available as part of the Gram-color stain set, (Product number 1.11885). It is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Informations sur la sécurité

• Pictogrammes: GHS02
• Mention d'avertissement: Warning
• Mentions de danger: H226
• Conseils de prudence: P210 - P233 - P240 - P241 - P242 - P243
• Classification des risques: Flam. Liq. 3
• Code de la classe de stockage: 3 - Flammable liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): 120.2 °F
• Point d'éclair (°C): 49 °C