07-2174

Anti-Heat Shock Protein 90α Antibody

from rabbit

• Synonyme(s) : heat shock protein 90kDa alpha (cytosolic), class A member 1, Renal carcinoma antigen NY-REN-38, heat shock 90kD protein 1, alpha, heat shock 90kD protein, alpha-like 4, heat shock 90kDa protein 1, alpha, heat shock protein HSP 90-alpha, Heat shock 86 kD
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human, rat, mouse
• Réactivité de l'espèce (prédite par homologie): canine (based on 100% sequence homology)
• Technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Numéro d'accès NCBI: NP_001017963
• Numéro d'accès UniProt: P07900
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: dog ... Hsp90Aa1(480438) ; human ... HSP90AA1(3320) ; mouse ... Hsp90Aa1(15519) ; rat ... Hsp90Aa1(299331)

Description

Description générale

The Heat Shock Protein 90 family is known as a group of molecular chaperones involved in the regulation of maturation, degradation, and folding transport for a select, yet varied group of proteins primarily comprised of signaling molecules, but also including tyrosine kinases, G-protein subunits, transcription factors, and telomerase. Heat Shock Protein 90 has two isoforms that share a homology of 76%; Heat Shock Protein 90α (HSP90α) and Heat Shock Protein 90β (HSP90β). HSP90α contains a charged sequence linker, a C-terminal domain, and N-terminal domain and a middle domain. It is induced by oxidative stress and has been observed in exosomes where it is secreted into the extracellular environment following translocation via TGFα stimulus. Extracellular HSP90α functions to facilitate the migration of both dermal and epidermal cells through its interaction with cell surface receptors CD91 and LRP1. HSP90α interaction with TERT is a necessary component of TERT holoenzyme complex assembly and stabilization. It has also been shown to interact with a variety of other proteins such as FNIP1, HSF1, TOM34, AHSA1, SMYD3, and DNAJC7.

Spécificité

This antibody recognizes Heat Shock Protein 90α at the N-terminus.

Immunogène

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of human Heat Shock Protein 90α.

Application

Detect the Heat Shock Protein 90α protein using this Anti-Heat Shock Protein 90α validated for use in WB, IP, ICC & IHC.

Research Category
Protein Trafficking

Research Sub Category
Chaperones

Western Blot (Snap i.d.) Analysis: 1 µg/mL from a previous lot detected Heat Shock Protein 90α on 10 µg of NIH/3T3 cell lysate.

Immunohistochemistry Analysis: 1:100 dilution from a previous lot detected Heat Shock Protein 90α in human prostate adenocarcinoma tissue.

Immunocytochemistry Analysis: 1:500 dilution from a previous ot detected Heat Shock Protein 90α in A431 cells.

Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated Heat Shock Protein 90A from HeLa cell lysate.

Qualité

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.0625 µg/mL of this antibody detected Heat Shock Protein 90α on 10 µg of HeLa cell lysate.

Description de la cible

~ 90 kDa observed

Forme physique

Format: Purified

Protein A

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
HeLa cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable