07-2051

Anti-PEMT Antibody (CT)

from rabbit

• Synonyme(s) : phosphatidylethanolamine N-methyltransferase
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Source biologique: rabbit
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human
• Technique(s): ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_009100.2
• Numéro d'accès UniProt: Q9UBM1
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PEMT(10400)

Description

Description générale

PEMT (phosphatidylethanolamine N-methyltransferase) is an an important enzyme for the synthesis of membrane phospholipids. PEMT converts phosphatidylethanolamine to phosphatidyl-choline by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17. Inhibition of hepatic PEMT activity has been associated with Alzheimer’s disease and schizophrenia.

Spécificité

Other homologies: Mouse (86% sequence homology) and Rat (73% sequence homology).

This antibody recognizes the C-terminal region of human PEMT in Jurkat cell lysates. The antibody easily detected PEMT in Jurkat and L6 lysates by Western blotting, but expression was weak or not detected in A431, HeLa, HEK293, PC12, or NIH/3T3 cell lysates using very dilute antibody (0.25 μg/mL, data not shown). Positive signals in immunocytochemistry using higher concentrations of antibody (2.5 μg/mL) detected PEMT in these cell lines, suggesting differential expression levels of this protein in various cell lines.

Immunogène

Epitope: C-terminus

The immunogen was a KLH-conjugated synthetic peptide corresponding to the C-terminal region of human PEMT.

Application

ELISA:
This antibody was reported by an outside laboratory to be suitable for ELISA

Immunocytochemistry:
HeLa, A431, and NIH/3T3 (previous page) cells incubated with anti-PEMT (C-terminus) stained positively on nuclei and endoplasmic reticulum (ER).

Research Category
Epigenetics & Nuclear Function

Neuroscience

Research Sub Category
Chromatin Biology

This PEMT antibody is validated for use in WB, ICC & ELISA for the detection of the PEMT protein.

Qualité

Western Blot Analysis:
1:1,000 dilution of this antibody detected PEMT on 10 µg of L6 lysate (below) and Jurkat lysate (not shown).

Description de la cible

~22 kDa

Forme physique

Format: Purified

Protein A chromatography

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Stockage et stabilité

Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance

Remarque sur l'analyse

Control
L6 and Jurkat lysates (Western blotting), HeLa, A431 cells (Immunocytochemistry)

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable