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Key Documents

07-1348

Sigma-Aldrich

Anti-phospho-PLK1 (Ser137) Antibody

from rabbit, purified by affinity chromatography

Synonyme(s) :

Polo-like kinase 1, Serine/threonine-protein kinase 13, cell cycle regulated protein kinase, polo (Drosophia)-like kinase, polo like kinase, polo-like kinase (Drosophila), polo-like kinase 1 (Drosophila)

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

human, mouse, rat

Réactivité de l'espèce (prédite par homologie)

bovine

Technique(s)

western blot: suitable

Numéro d'accès NCBI

NP_005021

Numéro d'accès UniProt

P53350

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

phosphorylation (pSer137)

Informations sur le gène

human ... PLK1(5347)

Description générale

Polo-like Kinase 1 (PLK1) is a serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Expressed PLK protein accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.

Spécificité

This antibody recognizes phosphorylated PLK1 at Ser137.

Immunogène

KLH-conjugated linear peptide corresponding to human phosphoylated PLK1 at and around Ser137.

Application

Detect phospho-PLK1 (Ser137) using this Anti-phospho-PLK1 (Ser137) Antibody validated for use in WB.
Research Category
Signaling
Research Sub Category
Kinases & Phosphatases
Western Blot (SNAP ID) Analysis: 0.5 µg/mL of this antibody detected PLK1 phosphorylated at Ser137 in human placenta tissue lysate.

Qualité

Evaluated by Western Blot in human placenta tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected PLK1 phosphorylated at Ser137 in lambda phosphatase treated and untreated human placenta tissue lysate.

Description de la cible

68 kDa

Forme physique

Affinity purified
Affinity purified rabbit serum in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Maintain refrigerated at 2-8°C for up to 12 months.

Remarque sur l'analyse

Control
Lambda phosphatase treated and untreated human placenta tissue lysate.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Juan Du et al.
Cell cycle (Georgetown, Tex.), 14(22), 3566-3579 (2015-12-15)
Polo-like kinase 1 (Plk1) is pivotal for proper mitotic progression, its targeting activity is regulated by precise subcellular positioning and phosphorylation. Here we assessed the protein expression, subcellular localization and possible functions of phosphorylated Plk1 (pPlk1(Ser137) and pPlk1(Thr210)) in mouse
Monika Raab et al.
Oncogene, 41(3), 372-386 (2021-11-12)
Polo-like kinase 1 (PLK1) is a crucial regulator of cell cycle progression. It is established that the activation of PLK1 depends on the coordinated action of Aurora-A and Bora. Nevertheless, very little is known about the spatiotemporal regulation of PLK1
Bin Peng et al.
Nucleic acids research, 49(13), 7554-7570 (2021-07-02)
Polo-like kinase 1 (PLK1) is a master kinase that regulates cell cycle progression. How its enzymatic activity is regulated in response to DNA damage is not fully understood. We show that PLK1 is enriched at double strand breaks (DSBs) within

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