05-591-AF647

Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Alexa Fluor^™ 647

clone SKB1, from mouse, ALEXA FLUOR^™ 647

• Synonyme(s) : RAC-alpha serine/threonine-protein kinase, PKB, PKB alpha, Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, RAC-PK-alpha
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Conjugué: ALEXA FLUOR^™ 647
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: SKB1, monoclonal
• Espèces réactives: human, mouse, rat
• Technique(s): immunocytochemistry: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NM_005163
• Numéro d'accès UniProt: P31749
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Akt1(11651)

Description

Description générale

RAC-alpha serine/threonine-protein kinase (EC 2.7.11.1; UniProt P31749; also known as PKB, PKB alpha, Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, RAC-PK-alpha) is encoded by the AKT1 (also known as PKB, RAC) gene (Gene ID 207) in human. AKTs (protein kinase Bs) are serine/threonine kinases that play an important role in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. There exit three types of AKTs (α, β, γ or AKT 1, 2, 3) sharing a high degree of homology, but differing slightly in the localization of their regulatory phosphorylation sites. AKT α is the predominant isoform in most tissues, whereas the highest expression of AKT β is observed in the insulin-responsive tissues, and AKT γ is abundant in brain tissue. Each AKT type is composed of three functionally distinct regions, including an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.

Spécificité

Clone SKB1 recognizes Akt1 and Akt2, but not Akt3, GST fusion proteins at 100 ng by immunoblotting analysis using the unconjugated antibody (Cat. No. 05-591).

Immunogène

Epitope: Amino acids 1-149

GST-fusion protein corresponding to residues 1-149 of human Akt1.

Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Akt/PKB in A431 cells.
The unconjugated antibody (Cat. No. 05-591) is shown to be suitable also for flow cytometry, immunocytochemistry, immunoprecipitation, and Western blotting applications.

Research Category
Apoptosis & Cancer

Research Sub Category
Kinases & Phosphatases

This Anti-Akt Antibody, PH Domain, clone SKB1, Alexa Fluor^™ 647 is validated for use in Immunocytochemistry for the detection of PKB.

Qualité

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Akt/PKB in HeLa cells.

Description de la cible

~60 kDa observed.

Forme physique

Protein G purified.

Purified mouse monoclonal IgG antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations légales

ALEXA FLUOR is a trademark of Life Technologies

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable