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Key Documents

05-591

Sigma-Aldrich

Anti-Akt/PKB Antibody, PH Domain, clone SKB1

clone SKB1, Upstate®, from mouse

Synonyme(s) :

Anti-AKT, Anti-PKB, Anti-PKB-ALPHA, Anti-PRKBA, Anti-RAC, Anti-RAC-ALPHA

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

affinity purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

SKB1, monoclonal

Espèces réactives

rat, human, mouse

Conditionnement

antibody small pack of 25 μg

Fabricant/nom de marque

Upstate®

Technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotype

IgG

Numéro d'accès NCBI

NM_005163

Numéro d'accès UniProt

P31749

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

mouse ... Akt1(11651)

Catégories apparentées

Description générale

Akt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.

Application

Detect Akt/PKB using this Anti-Akt/PKB Antibody, PH Domain, clone SKB1 validated for use in FC, IC, IP & WB.

Qualité

routinely evaluated by immunoblot on RIPA lysates from A431 cells

Description de la cible

60 kDa

Forme physique

Format: Purified
Protein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.02 M phosphate buffer, pH 7.6, 0.25M NaCl, 0.1% sodium azide and 30% glycerol.

Remarque sur l'analyse

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Evaluation of approaches to generation of tissue-specific knock-in mice.
Bayascas, JR; Sakamoto, K; Armit, L; Arthur, JS; Alessi, DR
The Journal of Biological Chemistry null
Regulation of AMP-activated protein kinase by multisite phosphorylation in response to agents that elevate cellular cAMP.
Hurley, RL; Barre, LK; Wood, SD; Anderson, KA; Kemp, BE; Means, AR; Witters, LA
The Journal of Biological Chemistry null
Important role of the LKB1-AMPK pathway in suppressing tumorigenesis in PTEN-deficient mice.
Huang X, Wullschleger S, Shpiro N, McGuire VA, Sakamoto K, Woods YL, McBurnie W et al.
The Biochemical Journal null
Heng-Liang Lin et al.
The Journal of pharmacology and experimental therapeutics, 323(1), 365-373 (2007-07-25)
Combretastatin A4 (CA4) is a drug that targets tumor vasculature to inhibit angiogenesis. Whether CA4 has a direct effect on gastric cancer is not known. We herein investigated the effect of CA4 on growth and metastasis of gastric cancer cells
HRT1 modulates vascular smooth muscle cell proliferation and apoptosis.
Wenli Wang, Chengyu Z Prince, Xing Hu, Matthew J Pollman
Biochemical and biophysical research communications null
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