05-253
Anti-ZAP-70 Antibody, clone 2F3.2
clone 2F3.2, Upstate®, from mouse
Synonyme(s) :
Anti-ADMIO2, Anti-SRK, Anti-STCD, Anti-STD, Anti-TZK, Anti-ZAP-70
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
2F3.2, monoclonal
Espèces réactives
human
Fabricant/nom de marque
Upstate®
Technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotype
IgG2a
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... ZAP70(7535)
Description générale
Note: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals.
Zeta-chain-associated protein kinase 70. A member of the protein tyrosine kinase family, ZAP-70 is normally expressed in T cells and natural killer cells and has a critical role in the initiation of T-cell signaling. ZAP-70 is expressed in T cells and tumors of T-cell lineage. A high level of ZAP-70 expression appears restricted to T-cell proliferative diseases and a subgroup of chronic lymphocytic leukemia (CLL). The ZAP-70 gene is in chromosome 2q12.
Spécificité
Recognizes human ZAP-70, does not recognize murine ZAP-70.
Immunogène
GST-fusion to tandem SH2 domains of human ZAP-70 corresponding to residues 1-254
Application
For testing in IHC, please see the following link (PDF): http://www.propathlab.com/pdf/focus_dec_2003.pdf
Research Category
Signaling
Signaling
Research Sub Category
Immunological Signaling
Immunological Signaling
This Anti-ZAP-70 Antibody, clone 2F3.2 is validated for use in FC, IH, IP, WB for the detection of ZAP-70.
Qualité
routinely evaluated on RIPA lysates prepared from Jurkat cells or Hut-78 cells
Description de la cible
70 kDa
Forme physique
Format: Purified
Protein G Purified
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Stockage et stabilité
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Remarque sur l'analyse
Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Expansion of cytotoxic effectors with lytic activity against autologous blasts from acute myeloid leukaemia patients in complete haematological remission.
Giovanni F Torelli, Anna Guarini, Gabriella Palmieri, Massimo Breccia, Antonella Vitale et al.
British Journal of Haematology null
S Pacini et al.
The Journal of biological chemistry, 273(32), 20487-20493 (1998-08-01)
The protein tyrosine kinase ZAP-70 plays a central role in T-cell activation. Following receptor engagement, ZAP-70 is recruited to the phosphorylated subunits of the T-cell antigen receptor (TCR). This event results in ZAP-70 activation and in association of ZAP-70 with
Nir Shani et al.
Blood, 113(15), 3530-3541 (2008-10-22)
The default pathway of cell-surface T-cell receptor (TCR) complex formation, and the subsequent transport to the membrane, is thought to entail endoplasmic reticulum (ER) localization followed by proteasome degradation of the unassembled chains. We show herein an alternative pathway: short
Alexander Kröber et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 24(6), 969-975 (2006-01-19)
Immunoglobulin heavy chain variable-region (VH) gene mutation status and zeta-associated protein 70 (ZAP-70) expression are correlated in chronic lymphocytic leukemia (CLL), but their concordance is variable. The goal of this study was to elucidate additional factors potentially characterizing their discordance.
Zachary B Katz et al.
Nature immunology, 18(1), 86-95 (2016-11-22)
Cell-surface-receptor pathways amplify weak, rare and local stimuli to induce cellular responses. This task is accomplished despite signaling components that segregate into nanometer-scale membrane domains. Here we describe a 'catch-and-release' mechanism that amplified and dispersed stimuli by releasing activated kinases
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